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Invitrogen

LIF Polyclonal Antibody, PeproTech®

2 Published Figures
1 Reference
View all (26) LIF antibodies

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LIF Polyclonal Antibody, PeproTech®

Product Details

500-P39-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human LIF
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930511

Product Specific Information

AA Sequence of recombinant protein: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human LIF. Anti-Human LIF-specific antibody was purified by affinity chromatography employing an immobilized Human LIF matrix.

Sandwich ELISA: To detect Human LIF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LIF.

Western Blot: To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LIF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cholinergic differentiation factor; D factor; differentiation inhibitory activity; differentiation-inducing factor; Differentiation-stimulating factor; hepatocyte-stimulating factor III; human interleukin in DA cells; Leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI; unnamed protein product

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Gene Aliases: CDF; DIA; HILDA; LIF; MLPLI

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UniProt ID: (Human) P15018

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Entrez Gene ID: (Human) 3976

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Function(s)
receptor binding cytokine activity leukemia inhibitory factor receptor binding protein binding growth factor activity
Process(es)
cell morphogenesis response to hypoxia blood vessel remodeling immune response embryo implantation cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation gene expression positive regulation of gene expression regulation of cell communication regulation of signaling cell differentiation macrophage differentiation lung development positive regulation of peptidyl-serine phosphorylation positive regulation of cell adhesion mediated by integrin somatic stem cell population maintenance positive regulation of MAPK cascade regulation of cell differentiation positive regulation of macrophage differentiation negative regulation of meiotic nuclear division positive regulation of transcription from RNA polymerase II promoter decidualization negative regulation of hormone secretion fibroblast proliferation positive regulation of fibroblast proliferation lung alveolus development muscle organ morphogenesis neuron development positive regulation of astrocyte differentiation leukemia inhibitory factor signaling pathway stem cell differentiation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of multicellular organismal process maternal process involved in female pregnancy lung vasculature development lung lobe morphogenesis trophoblast giant cell differentiation negative regulation of ERK1 and ERK2 cascade positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation receptor signaling pathway via STAT meiotic nuclear division positive regulation of STAT cascade regulation of multicellular organismal development
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