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Invitrogen
PDGF-BB Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
PDGF-BB Antibody (500-P47-100UG) in WB
Western Blot: To detect hPDGF-BB by Western Blot analysis PDGF-BB Polyclonal Antibody (Product # 500-P47-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P47-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human PDGF-BB
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-BB. Anti-Human PDGF-BB-specific antibody was purified by affinity chromatography employing an immobilized Human PDGF-BB matrix.
Sandwich ELISA: To detect Human PDGF-BB by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PDGF-BB (500-P47Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PDGF-BB.
Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: becaplermin; epididymis secretory sperm binding protein; FLJ12858; H-PDGF-BB; orf 2; PDGF subunit B; PDGF, B chain; PDGF-2; PDGFB; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis; unnamed protein product
Gene Aliases: c-sis; IBGC5; PDGF-2; PDGF2; PDGFB; SIS; SSV
UniProt ID: (Human) P01127
Entrez Gene ID: (Human) 5155
platelet-derived growth factor receptor binding
protein binding
collagen binding
growth factor activity
superoxide-generating NADPH oxidase activator activity
chemoattractant activity
identical protein binding
protein homodimerization activity
protein heterodimerization activity
receptor agonist activity
platelet-derived growth factor binding
angiogenesis
embryonic placenta development
positive regulation of endothelial cell proliferation
monocyte chemotaxis
positive regulation of glomerular filtration
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
heart development
positive regulation of cell proliferation
response to wounding
gene expression
regulation of gene expression
negative regulation of phosphatidylinositol biosynthetic process
negative regulation of platelet activation
positive regulation of macromolecule biosynthetic process
positive regulation of gene expression
negative regulation of gene expression
positive regulation of cell-substrate adhesion
smooth muscle adaptation
positive regulation of smooth muscle cell migration
peptidyl-tyrosine phosphorylation
hemopoiesis
positive regulation of cell migration
positive regulation of protein autophosphorylation
intracellular signal transduction
interleukin-18-mediated signaling pathway
positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway
cellular response to platelet-derived growth factor stimulus
paracrine signaling
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
positive regulation of blood vessel endothelial cell migration
positive regulation of mitotic nuclear division
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
positive regulation of smooth muscle cell proliferation
positive chemotaxis
positive regulation of chemotaxis
regulation of multicellular organismal process
positive regulation of cell division
positive regulation of protein kinase B signaling
cell chemotaxis
positive regulation of ERK1 and ERK2 cascade
cellular response to growth factor stimulus
cellular response to mycophenolic acid
positive regulation of glomerular mesangial cell proliferation
metanephric glomerular mesangial cell development
reactive oxygen species metabolic process
positive regulation of calcium ion import
positive regulation of hyaluronan biosynthetic process
positive regulation of intracellular signal transduction
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of vascular smooth muscle cell proliferation
positive regulation of vascular associated smooth muscle cell migration
negative regulation of vascular smooth muscle cell differentiation
positive regulation of vascular smooth muscle cell dedifferentiation
positive regulation of reactive oxygen species metabolic process
positive regulation of DNA biosynthetic process
positive regulation of metanephric mesenchymal cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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