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Invitrogen
CXCL1 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CXCL1 Antibody (500-P74BT-25UG) in WB
Western Blot: To detect Rat GRO/KC by Western Blot analysis CXCL1 Polyclonal Antibody, Biotin (Product # 500-P74BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat GRO/KC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P74BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat GRO/KC (CXCL1)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO/KC (CXCL1). Anti-Rat GRO/KC (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat GRO/KC by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat GRO/KC (500-P74) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat GRO/KC.
Western Blot: To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat GRO/KC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P74BT-1MG will be provided as 2 x 500 µg
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; gro; GRO a; GRO α; GROalpha; Growth-regulated alpha protein; GROα; NAP3; Platelet-derived growth factor-inducible protein KC
Gene Aliases: CINC-1; Cinc1; Cxcl1; Gro; Gro1; Scyb1
UniProt ID: (Rat) P14095
Entrez Gene ID: (Rat) 81503
response to amphetamine
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
response to heat
response to gamma radiation
positive regulation of sodium ion transport
neutrophil chemotaxis
response to estradiol
response to lipopolysaccharide
positive regulation of superoxide anion generation
regulation of cell proliferation
positive regulation of potassium ion transport
response to glucocorticoid
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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