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Invitrogen

BDNF Polyclonal Antibody, PeproTech®

6 Published Figures
8 References
View all (48) BDNF antibodies

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BDNF Polyclonal Antibody, PeproTech®

Product Details

500-P84-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
-

Neutralization (Neu)

-
View 5 publications 5 publications

Immunostaining (IS)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human/Murine/Rat BDNF
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930805

Product Specific Information

AA Sequence of recombinant protein: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BDNF matrix.

Sandwich ELISA: To detect Human/Murine/Rat BDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human/Murine/Rat BDNF (500-P84Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human/Murine/Rat BDNF.

Western Blot: To detect Human BDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Abrineurin; anorexia BDNF; BDNF; brain derived neurothrophic factor; brain derived neurotrophic factor; Brain-derived neurotrophic factor; growth factor; BDNF; H-BDNF; hBDNF1; hBDNF2A; hBDNF2B; hBDNF2C; hBDNF3; hBDNF4; hBDNF5; hBDNF6A; hBDNF6B; hBDNF7; neurotrophic factor; Neurotrophic factor BDNF precursor form; neurotrophin; proBDNF; unnamed protein product

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Gene Aliases: ANON2; BDNF; BULN2

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UniProt ID: (Human) P23560, (Rat) Q53YL8, (Mouse) P21237

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Entrez Gene ID: (Human) 627, (Rat) 24225, (Mouse) 12064

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Function(s)
receptor binding nerve growth factor receptor binding protein binding growth factor activity neurotrophin TRKB receptor binding neurotrophic factor
Process(es)
transmembrane receptor protein tyrosine kinase signaling pathway nervous system development axon guidance synapse assembly negative regulation of myotube differentiation positive regulation of neuron projection development nerve development brain-derived neurotrophic factor receptor signaling pathway positive regulation of brain-derived neurotrophic factor receptor signaling pathway nerve growth factor signaling pathway negative regulation of neuron apoptotic process collateral sprouting positive regulation of collateral sprouting neuron projection morphogenesis modulation of synaptic transmission positive regulation of synapse assembly regulation of protein localization to cell surface negative regulation of apoptotic signaling pathway ureteric bud development behavioral fear response response to hypoxia chronic inflammatory response mitochondrial electron transport, NADH to ubiquinone gamma-aminobutyric acid signaling pathway negative regulation of neuroblast proliferation axon target recognition learning or memory learning circadian rhythm feeding behavior neuron recognition response to light stimulus response to light intensity response to hormone response to auditory stimulus glutamate secretion response to fluoxetine response to activity dendrite development regulation of metabolic process neuron differentiation regulation of axon extension response to nutrient levels response to food negative regulation of synaptic transmission, GABAergic positive regulation of peptidyl-serine phosphorylation response to vitamin A response to tumor necrosis factor response to potassium ion mechanoreceptor differentiation response to drug fear response negative regulation of apoptotic process regulation of neuron apoptotic process positive regulation of neuron differentiation negative regulation of striated muscle tissue development regulation of retinal cell programmed cell death regulation of synaptic plasticity regulation of long-term neuronal synaptic plasticity positive regulation of long-term neuronal synaptic plasticity regulation of short-term neuronal synaptic plasticity axon extension inner ear development cognition positive regulation of sequence-specific DNA binding transcription factor activity response to electrical stimulus response to hyperoxia excitatory postsynaptic potential inhibitory postsynaptic potential negative regulation of cell death taste bud development cellular response to tumor necrosis factor cellular response to norepinephrine stimulus response to anesthetic dendrite extension negative regulation of neuron death response to L-glutamate response to nerve growth factor cellular response to nerve growth factor stimulus neuron projection extension positive regulation of glucocorticoid receptor signaling pathway cell-cell signaling cellular response to acid chemical
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