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Invitrogen
CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CXCL12 alpha (SDF-1 alpha) Antibody (500-P87ABT-500UG) in WB
Western Blot: To detect Human SDF-1alpha by Western Blot analysis CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin (Product # 500-P87ABT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P87ABT-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Bovine,
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 8.0 kDa Recombinant Human SDF-1alpha (CXCL12)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
500 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). Anti-Human SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human SDF-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SDF-1alpha (500-P87A) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human SDF-1alpha.
Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P87ABT-1MG will be provided as 2 x 500 µg
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; CXCL12; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF-1 delta; chemokine (C-X-C motif) ligand 12 transcript variant 5; SDF-1 epsilon; chemokine (C-X-C motif) ligand 12 transcript variant 6; SDF-1 gamma; chemokine (C-X-C motif) ligand 12 transcript variant 4; SDF-1 theta; chemokine (C-X-C motif) ligand 12 transcript variant 7; SDF1 alpha; SDF1a; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; unnamed protein product
Gene Aliases: CXCL12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1
UniProt ID: (Human) P48061
Entrez Gene ID: (Human) 6387
response to hypoxia
neuron migration
positive regulation of endothelial cell proliferation
cellular calcium ion homeostasis
chemotaxis
defense response
immune response
cell adhesion
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
axon guidance
blood circulation
regulation of actin polymerization or depolymerization
positive regulation of cell proliferation
adult locomotory behavior
response to mechanical stimulus
response to virus
T cell chemotaxis
telencephalon cell migration
positive regulation of cell migration
organ regeneration
killing of cells of other organism
positive regulation of dopamine secretion
integrin activation
chemokine (C-X-C motif) ligand 12 signaling pathway
CXCL12-activated CXCR4 signaling pathway
response to peptide hormone
positive regulation of neuron differentiation
positive regulation of cell adhesion
positive regulation of axon extension involved in axon guidance
positive regulation of chemotaxis
induction of positive chemotaxis
detection of temperature stimulus involved in sensory perception of pain
detection of mechanical stimulus involved in sensory perception of pain
regulation of calcium ion transport
cell chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
positive regulation of monocyte chemotaxis
positive regulation of calcium ion import
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of leukocyte tethering or rolling
positive regulation of vasculature development
response to ultrasound
cellular response to chemokine
positive regulation of T cell migration
negative regulation of dendritic cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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