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Invitrogen

CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech®

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View all (7) CXCL12 alpha antibodies

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CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P87ABT-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.25-1.0 µg/mL
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Bovine, Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 8.0 kDa Recombinant Human SDF-1alpha (CXCL12)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930826

Product Specific Information

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). Anti-Human SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human SDF-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SDF-1alpha (500-P87A) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human SDF-1alpha.

Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P87ABT-1MG will be provided as 2 x 500 µg

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; CXCL12; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF-1 delta; chemokine (C-X-C motif) ligand 12 transcript variant 5; SDF-1 epsilon; chemokine (C-X-C motif) ligand 12 transcript variant 6; SDF-1 gamma; chemokine (C-X-C motif) ligand 12 transcript variant 4; SDF-1 theta; chemokine (C-X-C motif) ligand 12 transcript variant 7; SDF1 alpha; SDF1a; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; unnamed protein product

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Gene Aliases: CXCL12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1

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UniProt ID: (Human) P48061

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Entrez Gene ID: (Human) 6387

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Function(s)
receptor binding cytokine activity integrin binding protein binding chemokine activity growth factor activity chemokine receptor binding CXCR chemokine receptor binding
Process(es)
response to hypoxia neuron migration positive regulation of endothelial cell proliferation cellular calcium ion homeostasis chemotaxis defense response immune response cell adhesion signal transduction G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway axon guidance blood circulation regulation of actin polymerization or depolymerization positive regulation of cell proliferation adult locomotory behavior response to mechanical stimulus response to virus T cell chemotaxis telencephalon cell migration positive regulation of cell migration organ regeneration killing of cells of other organism positive regulation of dopamine secretion integrin activation chemokine (C-X-C motif) ligand 12 signaling pathway CXCL12-activated CXCR4 signaling pathway response to peptide hormone positive regulation of neuron differentiation positive regulation of cell adhesion positive regulation of axon extension involved in axon guidance positive regulation of chemotaxis induction of positive chemotaxis detection of temperature stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception of pain regulation of calcium ion transport cell chemotaxis antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway positive regulation of monocyte chemotaxis positive regulation of calcium ion import negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of leukocyte tethering or rolling positive regulation of vasculature development response to ultrasound cellular response to chemokine positive regulation of T cell migration negative regulation of dendritic cell apoptotic process
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