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Invitrogen

IL-4 Polyclonal Antibody, Biotin, PeproTech®

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IL-4 Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P94BT-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat IL-4
3D Epitope / Immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL once reconstituted

Amount

50 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930874

Product Specific Information

AA Sequence of recombinant protein: MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-4. Anti-Rat IL-4-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-4 (500-P94) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-4.

Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P94BT-1MG will be provided as 2 x 500 µg

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IGG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; IL-4; cytokine; ILN; Interleukin; Interleukin4; lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: Il4e12

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UniProt ID: (Rat) P20096

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Entrez Gene ID: (Rat) 287287

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Function(s)
cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter microglial cell activation positive regulation of protein phosphorylation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment positive regulation of immunoglobulin production negative regulation of acute inflammatory response negative regulation of chronic inflammatory response positive regulation of leukocyte chemotaxis negative regulation of leukocyte activation immune response JAK-STAT cascade female pregnancy response to nutrient cholesterol metabolic process positive regulation of cell proliferation response to xenobiotic stimulus response to nematode regulation of proton transport response to selenium ion positive regulation of gene expression negative regulation of epithelial cell migration positive regulation of macroautophagy positive regulation of cell migration positive regulation of B cell proliferation B cell costimulation negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interleukin-10 production positive regulation of interleukin-13 production response to genistein response to cytokine interleukin-4-mediated signaling pathway positive regulation of T cell proliferation positive regulation of activated T cell proliferation T cell activation macrophage activation positive regulation of tyrosine phosphorylation of STAT protein defense response to protozoan myeloid dendritic cell differentiation negative regulation of macrophage activation positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation leukocyte activation positive regulation of MHC class II biosynthetic process response to ethanol positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade regulation of protein export from nucleus positive regulation of receptor-mediated endocytosis positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes negative regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response negative regulation of T cell activation positive regulation of B cell activation retina development in camera-type eye negative regulation of white fat cell proliferation cellular response to mercury ion positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of cold-induced thermogenesis neuroinflammatory response positive regulation of beta-amyloid clearance positive regulation of myoblast fusion positive regulation of cellular respiration positive regulation of establishment of endothelial barrier positive regulation of reactive oxygen species biosynthetic process negative regulation of complement-dependent cytotoxicity negative regulation of cellular response to transforming growth factor beta stimulus negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis positive regulation of B cell chemotaxis positive regulation of T-helper 2 cell cytokine production positive regulation of ATP biosynthetic process negative regulation of extrinsic apoptotic signaling pathway
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