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CD133 (Prominin-1) Monoclonal Antibody (13A4), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 49
CD133 (Prominin-1) Antibody (53-1331-80) in Flow
Knockout of CD133 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR924819_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD133 was performed using HT-29 WT control, cas9 control and CD133 Knock out cells with 0.5 µg Rat IgG1 kappa Isotype Control (eBRG1), eBioscience™ (Product # 14-4301-85, Fig.b) or 0.5 µg of ... View More
Product Details
53-1331-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
13A4
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 13A4 monoclonal antibody recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural and endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown. The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen but has been reported to work in canine/dog.
Applications Reported: This 13A4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13A4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: 5-transmembrane cell surface receptor; AC133 antigen; Antigen AC133; Antigen AC133 homolog; antigen CD133; CD107b; CD133; hematopoietic stem cell antigen; hematopoitic stem cell surface marker; hProminin; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; membrane glycoprotein; prominin 1.s1; Prominin-1; prominin-1.s2; prominin-like 1; Prominin-like protein 1; Prominin1; Retinitis pigmentosa 41, Cone-rod dystrophy 12; unnamed protein product
Gene Aliases: 4932416E19Rik; AC133; CD133; CORD12; MCDR2; MSTP061; Prom; Prom-1; PROM1; PROML1; RP41; STGD4
UniProt ID: (Human) Q6SV51, (Mouse) O54990
Entrez Gene ID: (Dog) 488816, (Human) 8842, (Mouse) 19126
retina layer formation
photoreceptor cell maintenance
retina morphogenesis in camera-type eye
camera-type eye photoreceptor cell differentiation
glomerular visceral epithelial cell differentiation
glomerular parietal epithelial cell differentiation
positive regulation of nephron tubule epithelial cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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