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Invitrogen

CD85k (ILT3) Monoclonal Antibody (ZM4.1), Alexa Fluor™ 488, eBioscience™

View all (19) LILRB4 (CD85k) antibodies

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Product Details

53-5139-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ZM4.1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802328

Product Specific Information

Description: The monoclonal antibody ZM4.1 recognizes ILT3, also known as CD85k. ILT3 is a member of the large family of ILT molecules (Immunologulin-like Transcripts, Leucocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT3 is a 60kDa transmembrane protein with two Ig domains and ITIMs. Expression is found on monocytes, macrophages, and dendritic cells. High levels of ILT3 expression is also found on plasmacytoid dendritic cells (pDCs). ILT3 expression by DCs has been shown to be required to induce Regulatory Tcells (tregs). Addition of ZM4.1, like ZM3.8, blocks the suppressive function of the Tregs in in vitro culture systems with allogenic DC's.

Applications Reported: This ZM4.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ZM4.1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: B4; CD85 antigen-like family member K; CD85k; ILT-3; Immunoglobulin-like transcript 3; leucocyte Ig-like receptor B4; Leukocyte immunoglobulin-like receptor 5; Leukocyte immunoglobulin-like receptor subfamily B member 4; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4; LILRB4; LIR-5; Monocyte inhibitory receptor HM18; unnamed protein product

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Gene Aliases: B4; CD85K; ILT-3; ILT3; LILRB4; LIR-5; LIR5

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UniProt ID: (Human) Q8NHJ6

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Entrez Gene ID: (Human) 11006

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Function(s)
fibronectin binding protein binding protein phosphatase binding protein tyrosine kinase inhibitor activity transmembrane receptor protein tyrosine kinase inhibitor activity receptor inhibitor activity inhibitory MHC class I receptor activity apolipoprotein binding
Process(es)
adaptive immune response immune system process tolerance induction positive regulation of T cell anergy negative regulation of T cell cytokine production immune response-regulating signaling pathway Fc receptor mediated inhibitory signaling pathway cell surface receptor signaling pathway cytokine-mediated signaling pathway receptor internalization negative regulation of chemokine production negative regulation of interferon-gamma production negative regulation of interleukin-1 beta production negative regulation of interleukin-10 production negative regulation of interleukin-2 production negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production negative regulation of T cell proliferation negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of CD8-positive, alpha-beta T cell differentiation negative regulation of MAPK cascade negative regulation of cytotoxic T cell differentiation positive regulation of regulatory T cell differentiation negative regulation of osteoclast differentiation negative regulation of activated T cell proliferation negative regulation of T cell receptor signaling pathway negative regulation of IP-10 production interleukin-10-mediated signaling pathway negative regulation of monocyte activation negative regulation of cytokine production involved in inflammatory response negative regulation of protein localization to nucleus negative regulation of intracellular signal transduction negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of T cell costimulation
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