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Invitrogen
SARS-CoV-2 Spike Protein (RBD) Chimeric Recombinant Rabbit Monoclonal Antibody (P05DHuRb), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 1
SARS-CoV-2 Spike Protein (RBD) Chimeric Antibody (53-6491-82) in Flow
HEK 293 cells were transfected with a vector encoding full length spike protein from SARS CoV2 (pink histogram) or left untransfected (blue histogram) and then stained with 1 µg of Anti-SARS CoV2 Spike Protein RBD Recombinant Rabbit Chimera Monoclonal Antibody, Alexa Fluor 488 (Product # 53-6491-82). Viable cells were used for analysis as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18)... View More
Product Details
53-6491-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
P05DHuRb
Immunogen
This antibody was developed using computationally derived variants of SARS-CoV antibody structures and is shown to bind Spike Protein S1 subunit (RBD) of SARS CoV2
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer/PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This antibody has a human Fab with a kappa light chain and Rabbit Fc. It recognizes the RBD domain of SARS CoV2 Spike protein.
Applications Tested: This P05DHuRb Alexa Fluor™ 488 conjugate has been tested by flow cytometric analysis of HEK 293 cells transfected with a vector encoding full length spike protein from SARS CoV2. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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