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Invitrogen
KLRG1 Monoclonal Antibody (13F12F2), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 4
KLRG1 Antibody (53-9488-42) in Flow
LEFT: Staining of normal human peripheral blood cells with Anti-Human CD28 eFluor® 450 (Product # 48-0289-42) and Anti-Human KLRG1 Alexa Fluor® 488. CD8+ cells in the lymphocyte gate were used for analysis. RIGHT: Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE-eFluor® 610 (Product # 61-0567-42) and Anti-Human KLRG1 Alexa Fluor® 488. Cells in the lymphocyte gate were used for analysis.
Product Details
53-9488-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
13F12F2
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain. This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells. KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function. Cadherin-E, -N, and -R are ligands for KLRG1. Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience.
The clones 13F12F2 and 13A2 appear to recognize a similar epitope based on cross-blocking studies.
Applications Reported: This 13F12F2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13F12F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: C-type lectin domain family 15 member A; ITIM-containing receptor MAFA-L; Killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; lectin-like type II transmembrane protein; MAFA-like receptor; Mast cell function-associated antigen; mast cell function-associated antigen (ITIM-containing); natural killer cell receptor; predicted type II transmembrane glycoprotein; type II integral membrane glycoprotein; similar to C-type animal lectins; unnamed protein product
Gene Aliases: 2F1; CLEC15A; KLRG1; MAFA; MAFA-2F1; MAFA-L; MAFA-LIKE; MAFAL
UniProt ID: (Human) Q96E93
Entrez Gene ID: (Human) 10219
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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