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Invitrogen
COX2 Monoclonal Antibody (COX 229), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 2
COX2 Antibody (53-9725-82) in Flow
Normal human peripheral blood cells were stimulated for 5 hours with LPS (Product # 00-4976-03) and then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD14 Monoclonal Antibody, APC (Product # 17-0149-42) and 0.125 µg Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or COX2 Monoclonal Antibody, Alexa Fluor 488 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
53-9725-82
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
COX 229
Immunogen
Synthetic peptide from human COX2
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This COX 229 monoclonal antibody recognizes human COX2 also known as PTGS2. This antibody has been reported to cross-react with the mouse and rat COX2. For optimal performance we recommend using this antibody with the Foxp3 / Transcription Factor Staining Buffer Set and protocol.
Applications Reported: This COX 229 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This COX 229 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2 splice variant; cyclooxygenase 2b; Cyclooxygenase-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); unnamed protein product
Gene Aliases: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2; PTGS2
UniProt ID: (Human) P35354
Entrez Gene ID: (Human) 5743
peroxidase activity
prostaglandin-endoperoxide synthase activity
protein binding
oxidoreductase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
enzyme binding
heme binding
protein homodimerization activity
metal ion binding
dioxygenase activity
prostaglandin biosynthetic process
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
prostaglandin metabolic process
response to oxidative stress
embryo implantation
regulation of blood pressure
response to nematode
response to selenium ion
positive regulation of vascular endothelial growth factor production
cyclooxygenase pathway
lipoxygenase pathway
positive regulation of prostaglandin biosynthetic process
positive regulation of fever generation
prostaglandin secretion
regulation of cell proliferation
long-chain fatty acid biosynthetic process
negative regulation of apoptotic process
positive regulation of nitric oxide biosynthetic process
decidualization
regulation of inflammatory response
brown fat cell differentiation
cellular response to hypoxia
cellular response to non-ionic osmotic stress
cellular response to fluid shear stress
positive regulation of transforming growth factor beta production
positive regulation of cell migration involved in sprouting angiogenesis
positive regulation of fibroblast growth factor production
positive regulation of brown fat cell differentiation
positive regulation of platelet-derived growth factor production
cellular oxidant detoxification
regulation of neuroinflammatory response
negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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