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Vinculin Monoclonal Antibody (7F9), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 9
Vinculin Antibody (53-9777-82) in ICC/IF
Knockout of Vinculin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR948202_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on HeLa wild type cells (panel a,d), HeLa Cas9 control cells (panel b,e) and HeLa Vinculin KO cells (panel c, f). Cells were fixed, permeabilized, and labeled with Vinculin Monoclonal Antibody (7F9), Alexa Fluor™ 488, eBioscience™ (Product # 53-9777-82, 1:100 dilution), followed by Donkey anti-Mouse IgG (H+... View More
Product Details
53-9777-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
7F9
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.
Applications Reported: This 7F9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This 7F9 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 114; epididymis secretory sperm binding protein; meta-vinculin; Metavinculin; metavinculin {ECO:0000250|UniProtKB:P18206}; MV; RP11-178G16.3; unnamed protein product; vcl {ECO:0000250|UniProtKB:P18206}; vinc; Vinculin; vinculin {ECO:0000250|UniProtKB:P18206}
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL; VINC
UniProt ID: (Human) P18206, (Mouse) Q64727, (Rat) P85972
Entrez Gene ID: (Human) 7414, (Mouse) 22330, (Rat) 305679
morphogenesis of an epithelium
cell adhesion
cell-matrix adhesion
lamellipodium assembly
regulation of cell migration
negative regulation of cell migration
adherens junction assembly
protein localization to cell surface
maintenance of blood-brain barrier
apical junction assembly
axon extension
regulation of focal adhesion assembly
platelet aggregation
epithelial cell-cell adhesion
regulation of establishment of endothelial barrier
regulation of protein localization to cell-cell adherens junction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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