Bioss

5HT4 Receptor Polyclonal Antibody

Product Image Gallery

5HT4 Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 5HT4 Receptor Polyclonal Antibody (bs-2127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) by operating according instructions and DAB staining.

5HT4 Receptor Antibody in Western Blot (WB) — L1 rat brain, L2 rat heart lysates probed (bs-2127R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted and observed band size: 43 kDa.

Product Details

BS-2127R

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200	-
Immunohistochemistry (Paraffin) (IHC (P))	1:400	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide derived from human 5-HTR4, amino acids 21-120. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 50% glycerol, 1% BSA
Contains	0.09% sodium azide
Storage conditions	-20°C

Target Information

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described, but the full-length nature of some transcript variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 5 hydroxytryptamine ( serotonin) receptor 4; 5-HT-4; 5-HT4 receptor; 5-HT4 receptor (two splicing variants L and S); 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled; 5-hydroxytryptamine receptor 4; 5HT4; 5HT4 receptor; cardiac 5-HT4 receptor; Serotonin receptor 4

Gene Aliases: 5-HT-4; 5-HT4; 5-HT4R; 5-HT<4L>; 5HTR4; HTR4

UniProt ID: (Human) Q13639, (Rat) Q62758, (Mouse) P97288

Entrez Gene ID: (Human) 3360, (Rat) 25324, (Mouse) 15562

Molecular Function: G-protein coupled receptor