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          • Primary Antibodies ›
          • PTEN Antibodies

          Rockland

          Phospho-PTEN-P1 (Ser29, Thr32) Polyclonal Antibody

          View all (23) PTEN antibodies

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          Cite Phospho-PTEN-P1 (Ser29, Thr32) Polyclonal Antibody

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          Phospho-PTEN-P1 (Ser29, Thr32) Polyclonal Antibody

          Product Details

          600-401-859

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500- 1:2,000
          -

          ELISA (ELISA)

          1:20,000-1:80,000
          -

          Immunoprecipitation (IP)

          1:500-1:2,000
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, Chimpanzee, Human, Mouse, Rhesus monkey, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 223-237 of human PTEN protein.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.87 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          This affinity-purified antibody is directed against the phosphorylated form of human PTEN protein at the pS29 and pT32 residues. Reactivity occurs against human PTEN pS29/pT32 protein and the antibody is specific for the phosphorylated form of the proteins. Approximately 3% reactivity is observed against the non-phosphorylated form of the immunizing peptide. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse and dog sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

          Target Information

          PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Bioinformatics

          Protein Aliases: homolog of human mutated in multiple advanced cancers; Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; N-terminal extended PTEN isoform; uses an alternative ATT initiation codon in PTEN 5'UTR; phosphatidylinositol-3,4,5-trisphosphate3-phosphatase; phosphatase and tensin; Phosphatase and tensin homolog; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); phosphatase and tensin homolog deleted on chromosome ten; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 5; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 6; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 7; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, isoform 21; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, isoform 22; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; protein tyrosine phosphatase and tensin homolog; protein tyrosine phosphatase and tensin-like protein; PTEN; pten tumor suppressor; PTEN variant PTEN-K267Rfs*9; PTEN variant PTEN-L247*; PTEN variant PTEN-R234Afs*11; PTENepsilon; TEP1; unnamed protein product

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          Gene Aliases: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; Mmac; MMAC1; PTEN; PTEN1; PTENbeta; PTENgama; rCG_47874; TEP1

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          UniProt ID: (Dog) P60483, (Human) P60484, (Rat) O54857, (Mouse) O08586

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          Entrez Gene ID: (Dog) 403832, (Human) 5728, (Bovine) 540786, (Rhesus monkey) 705121, (Rat) 50557, (Mouse) 19211

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          Function(s)
          phosphatidylinositol-3-phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity protein tyrosine/serine/threonine phosphatase activity anaphase-promoting complex binding phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity protein kinase binding PDZ domain binding identical protein binding inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity ubiquitin-specific protease binding phosphoprotein phosphatase activity protein binding beta-catenin binding lipid binding hydrolase activity phosphatase activity enzyme binding inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity phosphatidylinositol phosphate phosphatase activity molecular function inhibitor activity ubiquitin ligase activator activity magnesium ion binding platelet-derived growth factor receptor binding protein phosphatase
          Process(es)
          angiogenesis regulation of B cell apoptotic process protein dephosphorylation apoptotic process neuron-neuron synaptic transmission central nervous system development heart development learning or memory positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of epithelial to mesenchymal transition regulation of neuron projection development cell migration dentate gyrus development central nervous system neuron axonogenesis negative regulation of cell migration adult behavior negative regulation of myelination regulation of protein stability regulation of synaptic transmission, GABAergic central nervous system myelin maintenance regulation of myeloid cell apoptotic process negative regulation of peptidyl-serine phosphorylation multicellular organismal response to stress social behavior peptidyl-tyrosine dephosphorylation maternal behavior negative regulation of apoptotic process protein kinase B signaling endothelial cell migration locomotor rhythm negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell size inositol phosphate dephosphorylation phosphatidylinositol dephosphorylation negative regulation of axon regeneration cardiac muscle tissue development forebrain morphogenesis negative regulation of epithelial cell proliferation negative regulation of axonogenesis protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of keratinocyte migration negative regulation of focal adhesion assembly negative regulation of protein kinase B signaling rhythmic synaptic transmission negative regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway synapse maturation prepulse inhibition male mating behavior long-term synaptic potentiation prostate gland growth dendritic spine morphogenesis negative regulation of dendritic spine morphogenesis negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to electrical stimulus cellular response to hypoxia negative regulation of ribosome biogenesis negative regulation of cell aging negative regulation of excitatory postsynaptic potential presynaptic membrane assembly postsynaptic density assembly negative regulation of wound healing, spreading of epidermal cells positive regulation of TRAIL-activated apoptotic signaling pathway positive regulation of ubiquitin protein ligase activity negative regulation of vascular smooth muscle cell proliferation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of excitatory postsynaptic potential negative regulation of synaptic vesicle clustering osteoblast differentiation lipid metabolic process phosphatidylinositol biosynthetic process spindle assembly involved in female meiosis nervous system development synapse assembly locomotory behavior regulation of signal transduction positive regulation of macromolecule metabolic process negative regulation of cell communication negative regulation of neuron projection development negative regulation of signaling regulation of cell differentiation negative regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of organ growth cell motility negative regulation of developmental process negative regulation of cellular component organization negative regulation of multicellular organismal process regulation of cell cycle regulation of protein kinase B signaling regulation of biological quality positive regulation of intracellular signal transduction negative regulation of cell cycle G1/S phase transition negative regulation of cellular senescence negative regulation of protein phosphorylation aging response to nutrient memory response to glucose response to organic substance response to inorganic substance response to zinc ion response to organic cyclic compound dephosphorylation neuron projection development negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle response to estradiol regulation of cellular component size response to ATP cellular response to decreased oxygen levels response to drug positive regulation of apoptotic process response to ethanol response to arsenic-containing substance platelet-derived growth factor receptor signaling pathway regulation of axon regeneration brain morphogenesis negative regulation of phagocytosis long term synaptic depression positive regulation of apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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