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CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Super Bright™ 436, eBioscience™
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Additional Information:
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-Flow-20170216130641.jpg?time=20220826 "CD90 (Thy-1) Antibody in Flow Cytometry (Flow)")
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD90 (Thy-1) Antibody (62-0909-42) in Flow")
Product Details
62-0909-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio5E10 (5E10)
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kDa receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of the HEL cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy-1 T-cell antigen; unnamed protein product
Gene Aliases: CD90; CDw90; THY1
UniProt ID: (Human) P04216
Entrez Gene ID: (Human) 7070
angiogenesis
regulation of cell-matrix adhesion
regulation of immune system process
positive regulation of cellular extravasation
negative regulation of protein kinase activity
cytoskeleton organization
cell adhesion
cell surface receptor signaling pathway
integrin-mediated signaling pathway
cell-cell signaling
regulation of cell migration
negative regulation of cell migration
heterotypic cell-cell adhesion
receptor clustering
positive regulation of GTPase activity
positive regulation of cell adhesion
retinal cone cell development
focal adhesion assembly
negative regulation of axonogenesis
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of focal adhesion assembly
negative regulation of neuron projection regeneration
cell-cell adhesion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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