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Invitrogen
CD4 Monoclonal Antibody (YKIX302.9), Super Bright™ 436, eBioscience™
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FIGURE: 1 / 3
CD4 Antibody (62-5040-42) in Flow
Frozen canine peripheral blood cells were stained with CD5 Monoclonal Antibody, PE (Product # 12-5050) and Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321) (left) or CD4 Monoclonal Antibody, Super Bright 436 (Product # 62-5040) (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993).
Product Details
62-5040-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
YKIX302.9
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on a majority of thymocytes, a subpopulation of mature T cells and dendritic cells. In dogs, CD4 is also highly expressed on neutrophils. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role in T cell development and homeostasis.
This YKIX302.9 monoclonal antibody has been reported to block MHC II-restricted T cell responses in vivo and in vitro.
Applications Reported: This YKIX302.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YKIX302.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal canine peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Gene Aliases: CD4
UniProt ID: (Dog) P33705
Entrez Gene ID: (Dog) 403931
adaptive immune response
induction by virus of host cell-cell fusion
cell adhesion
T cell differentiation
macrophage differentiation
maintenance of protein location in cell
positive regulation of I-kappaB kinase/NF-kappaB signaling
T cell selection
positive regulation of monocyte differentiation
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
positive regulation of viral entry into host cell
regulation of T cell activation
regulation of calcium ion transport
cellular response to granulocyte macrophage colony-stimulating factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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