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Proteintech
CD20 Monoclonal Antibody (2H7)
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FIGURE: 1 / 2
CD20 Antibody (65085-1-IG) in Flow
1X10^6 Human PBMCs were surface stained with 0.2 µg Anti-Human CD20 (Product # 65085-1-Ig) (Clone: 2H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1,000 (red), or stained with 0.2 µg Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1,000 (blue). Cells were not fixed. Lymphocytes are gated.
Product Details
65085-1-IG
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
2H7
Immunogen
Human tonsillar B cells
Conjugate
Form
Liquid
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Target Information
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969; unnamed protein product
Gene Aliases: B1; Bp35; CD20; CVID5; FMC7; LEU-16; MS4A1; S7
UniProt ID: (Human) P11836
Entrez Gene ID: (Human) 931
store-operated calcium entry
humoral immune response
cell surface receptor signaling pathway
response to bacterium
B cell differentiation
B cell proliferation
B cell activation
B cell receptor signaling pathway
protein tetramerization
calcium ion import into cytosol
positive regulation of calcium ion import across plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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