Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 66240-1-IG) (beta Tubulin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staining with CoraLite555-Phalloidin.

beta Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta Tubulin Antibody in Western Blot (WB)

beta Tubulin Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image beta Tubulin Monoclonal Antibody (1D4A4)

Product Details

66240-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000-1:100,000

View 254 publications 254 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 18 publications 18 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Not applicable (N/A)

View 9 publications 9 publications

Product Specifications

Species Reactivity

C. elegans, Human, Mouse, Pig, Rat, Zebrafish

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1D4A4

Immunogen

beta Tubulin Fusion Protein Ag0117 (44-259 aa encoded by BC000748)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.43 mg/mL

214.5 µg

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LERISVYYN EASSHKYVPR AILVDLEPGT MDSVRSGAFG HLFRPDNFIF GQSGAGNNWA KGHYTEGAEL VDSVLDVVRK ECENCDCLQG FQLTHSLGGG TGSGMGTLLI SKVREEYPDR IMNTFSVVPS PKVSDTVVEP YNATLSIHQL VENTDETYCI DNEALYDICF RTLKLATPTY GDLNHLVSAT MSGVTTSLRF PGQLNADLRK LAVNMVP (44-259 aa encoded by BC000748)

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta 5-tubulin; beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); epididymis secretory sperm binding protein; HSA10p15 beta-tubulin 4Q; M40 antibody; MGC8685; microtubule protein; Neuron-specific class III beta-tubulin; similar to beta tubulin; T beta-15; TUBB; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B; tubulin, beta 3; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 6; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 5; unnamed protein product; XTP3TPATP1; zbetaI

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Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CDCBM7; CFEOM3; CFEOM3A; CSCSC1; DYT4; fb40f09; FEOM3; FPVEPD; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; MACTHC1; OK/SW-cl.56; OOMD; OOMD2; OZEMA2; PMGYSA; RGD1305887; RGD1309427; si:ch211-122i22.4; si:ch211-9a18.2; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8; wu:fb40f09; zgc:112515; zgc:123194