|ELISA (ELISA)||25-500 ng/ml|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||1:250-1:1000|
|Tested Species reactivity||Tag|
|Host / Isotype||Mouse / IgG|
|Immunogen||Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
By Western blot, MA1-135 detects both N-terminal and C-terminal His-tagged proteins.
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
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