|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-5 ug/ml|
|Western Blot (WB)||See 11 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Zebrafish , Human , Mouse , Chicken|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide containing six His residues (6xHis).|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.