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Invitrogen

ApoA1 Recombinant Rabbit Monoclonal Antibody (19H20L19)

2 Published Figures
8 References
View all (59) ApoA1 antibodies

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ApoA1 Recombinant Rabbit Monoclonal Antibody (19H20L19)

Product Details

701239

Applications
Tested Dilution
Publications

Western Blot (WB)

2-4 µg/mL
View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

19H20L19

Immunogen

Peptide corresponding to amino acids 127-142 of human Apolipoprotein A1
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532437

Product Specific Information

This antibody is predicted to react with mouse and non-human primate based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

By sandwich ELISA, 701239 can be used as a coating antibody with each of the following mouse monoclonal detection antibodies: Product # MA5-14668, MA5-14670, or MA5-14732, to generate a matched pair. With each of these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, is detected. 701239 can be used to capture Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with 701239, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; apolipoprotein A-1; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; epididymis secretory sperm binding protein; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; proapo-A-I protein; proapolipoprotein; Udpgt; Ugt1; Ugt1a7; unnamed protein product

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Gene Aliases: AMYLD3; apo(a); apoA-I; APOA1; HPALP2

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UniProt ID: (Human) P02647, (Rat) P04639

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Entrez Gene ID: (Human) 335, (Rat) 25081

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Function(s)
beta-amyloid binding receptor binding lipid transporter activity protein binding phospholipid binding high-density lipoprotein particle binding lipid binding cholesterol binding enzyme binding heat shock protein binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding protein homodimerization activity chemorepellent activity receptor agonist activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding lipoprotein particle binding intermembrane cholesterol transfer activity phospholipid transporter activity cholesterol transporter activity phosphatidylcholine binding lipase inhibitor activity apolipoprotein
Process(es)
endothelial cell proliferation negative regulation of cytokine production involved in immune response lipid metabolic process phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process lipid transport transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway integrin-mediated signaling pathway steroid metabolic process cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol efflux negative regulation of very-low-density lipoprotein particle remodeling protein oxidation peptidyl-methionine modification lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development regulation of Cdc42 protein signal transduction negative regulation of interleukin-1 beta production cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion beta-amyloid formation high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport negative regulation of inflammatory response positive regulation of phagocytosis protein stabilization negative chemotaxis vitamin transport positive regulation of stress fiber assembly acylglycerol homeostasis phospholipid homeostasis negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis ERK1 and ERK2 cascade cholesterol import cellular response to lipoprotein particle stimulus positive regulation of cholesterol metabolic process positive regulation of substrate adhesion-dependent cell spreading positive regulation of phospholipid efflux cellular response to beta-amyloid regulation of protein phosphorylation negative regulation of cytokine secretion involved in immune response response to nutrient protein localization positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process peripheral nervous system axon regeneration phospholipid transport organ regeneration axon regeneration very-low-density lipoprotein particle remodeling response to drug response to estrogen positive regulation of fatty acid biosynthetic process phosphatidylcholine metabolic process negative regulation of interleukin-1 beta secretion positive regulation of lipoprotein lipase activity positive regulation of hydrolase activity negative regulation of hydrolase activity positive regulation of transferase activity negative regulation of lipase activity
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