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Invitrogen
EEF2 Recombinant Rabbit Monoclonal Antibody (20H2L1)
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FIGURE: 1 / 4
EEF2 Antibody (702807) in ICC/IF
For immunofluorescence analysis, HCT116 cells were fixed and permeabilized for detection of endogenous EEF2 using Anti- EEF2 Recombinant Rabbit Monoclonal Antibody (Product # 702807, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EEF2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3693... View More
Product Details
702807
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
20H2L1
Immunogen
Protein is corresponding to human EEF2 (aa241-aa340)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Bovine, Sheep
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EF-2; Elongation factor 2; polypeptidyl-tRNA translocase
Gene Aliases: EEF-2; EEF2; EF-2; EF2; SCA26
UniProt ID: (Human) P13639
Entrez Gene ID: (Human) 1938
hematopoietic progenitor cell differentiation
response to ischemia
skeletal muscle contraction
translational elongation
aging
glial cell proliferation
response to fluoxetine
peptidyl-diphthamide biosynthetic process from peptidyl-histidine
response to estradiol
response to endoplasmic reticulum stress
skeletal muscle cell differentiation
response to hydrogen peroxide
response to ethanol
positive regulation of translation
response to folic acid
cell-cell adhesion
cellular response to brain-derived neurotrophic factor stimulus
positive regulation of cytoplasmic translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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