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FIGURE: 1 / 10
SUZ12 Antibody (711520) in ICC/IF
For immunofluorescence analysis, Ntera2 cells were fixed and permeabilized for detection of endogenous SUZ12 using Anti- SUZ12 Recombinant Rabbit Superclonal™ Antibody (Product # 711520, 2µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SUZ12 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S... View More
Product Details
711520
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human SUZ12 [aa545-aa725]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig, and Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
SUZ12 (polycomb protein SUZ12) is a zinc finger gene and has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. SUZ12 is also a component of the PRC2/EED-EZH2 complex which methylates Lys-9 (H3K9me) and Lys-27 (H3K27me) of histone H3 leading to transcriptional repression of the affected target gene. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ChET 9 protein; Chromatin precipitated E2F target 9 protein; Joined to JAZF1 protein; KIAA0160; Polycomb protein SUZ12; Suppressor of zeste 12 protein homolog; unnamed protein product
Gene Aliases: CHET9; IMMAS; JJAZ1; KIAA0160; SUZ12
UniProt ID: (Human) Q15022
Entrez Gene ID: (Cynomolgus monkey) 102114791, (Human) 23512
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
chromatin binding
RNA binding
protein binding
enzyme activator activity
zinc ion binding
chromatin DNA binding
sequence-specific DNA binding
metal ion binding
histone H3K9me2/3 reader activity
lncRNA binding
promoter-specific chromatin binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
cell proliferation
positive regulation of cell proliferation
negative regulation of cell differentiation
oligodendrocyte differentiation
random inactivation of X chromosome
facultative heterochromatin formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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