Search
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
Caspase 1 Antibody (711892) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP-1 (Lane 1), HT-29 (Lane 2) and HT-29 treated with IFN-gamma (10 ng/mL for 24 hrs) (Lane 3). The blot was probed with Anti-Caspase 1 Recombinant Rabbit Superclonal™ Antibody (Product # 711892, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~45 kDa band corresponding to Caspase 1 was observed in the cell lines tested.
Product Details
711892
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
24HCLC
Immunogen
Protein corresponding to human Caspase 1 [aa120-aa297]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1; Caspase1; cysteine protease; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; unnamed protein product
Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45
UniProt ID: (Human) P29466
Entrez Gene ID: (Human) 834
endopeptidase activity
cysteine-type endopeptidase activity
protein binding
peptidase activity
cysteine-type peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
hydrolase activity
enzyme binding
kinase binding
cytokine binding
identical protein binding
CARD domain binding
negative regulation of transcription from RNA polymerase II promoter
plasma membrane repair
pattern recognition receptor signaling pathway
proteolysis
apoptotic process
inflammatory response
signal transduction
osmosensory signaling pathway
protein secretion
protein autoprocessing
protein catabolic process
response to lipopolysaccharide
positive regulation of interleukin-1 beta production
positive regulation of interleukin-18 production
interleukin-18-mediated signaling pathway
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
icosanoid biosynthetic process
ceramide biosynthetic process
regulation of inflammatory response
positive regulation of inflammatory response
protein maturation
defense response to virus
protein poly-ADP-ribosylation
pyroptosis
interleukin-1-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to interferon-gamma
cytokine precursor processing
signaling receptor ligand precursor processing
AIM2 inflammasome complex assembly
pyroptotic cell death
positive regulation of tumor necrosis factor-mediated signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support