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FIGURE: 1 / 2
GST Antibody (740007MHRP) in WB
Western blot was performed using GST tag Recombinant Mouse monoclonal antibody (GST 3-4C), HRP (Product # 740007MHRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: Untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), H3-GST, 40 µg (Lane 3), H3-GST, 30 µg (Lane 4), H3-GST, 20 µg (Lane 5), GST-p65, 40 µg (Lane 6), GST-p65, 30 µg (Lane 7), GST-p65, 20 µg (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then trans... View More
Product Details
740007MHRP
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
GST 3-4C
Immunogen
GST protein from Schistosoma japonicum expressed from the pGEX vector.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with proprietary stabilizer
Contains
proprietary preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
GST Tag recombinant mouse monoclonal antibody conjugated with HRP specifically recognizes GST Tag at both C and N-terminus of proteins.
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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