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c-Rel Antibody (740087TP488) in WB
Western blot was performed using c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5), Alexa Fluor™ Plus 488 (Product # 740087TP488) and a ~65 kDa band corresponding to c-Rel was observed across cell lines tested. Whole cell extracts (30 µg lysate) of EL4 (Lane 1), RAW 264.7 (Lane 2) and NIH/3T3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel, (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001) and blocked... View More
Product Details
740087TP488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1RELAH5
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008 Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This 1RELAH5 antibody has been tested by western blot and flow cytometric analysis of EL4 cells. This may be used for flow cytometry at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCD541; C-Rel protein; C-Rel proto-oncogene protein; gemin 1; gemin-1; proto-oncogene c-Rel; SMA; SMA1; SMA2; SMA3; SMA4; SMN; SMN2; SMNT
Gene Aliases: c-Rel
Entrez Gene ID: (Mouse) 19696
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
cytokine production
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
inflammatory response
I-kappaB kinase/NF-kappaB signaling
negative regulation of gene expression
negative regulation of interferon-beta production
response to cytokine
NIK/NF-kappaB signaling
positive regulation of interleukin-12 biosynthetic process
innate immune response
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of neuron death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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