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Invitrogen
RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC)
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FIGURE: 1 / 5
RUNX1 Antibody (740114T) in WB
Western blot was performed using RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC) (Product # 740114T) and ~50 kDa band corresponding to RUNX1 was observed in HEL 92.1.7 and Jurkat as compared to BT-549, T-47D and IMR-32 which is reported to be negative for RUNX1 expression. Nuclear enriched extracts (1% SDS) (30 µg lysate) of HEL 92.1.7 (Lane 1), Jurkat (Lane 2), BT-549 (Lane 3), T-47D (Lane 4) and IMR-32 (Lane 5) were electrophoresed using Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transf... View More
Product Details
740114T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
RXDMC
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor
Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha; RUNX1
UniProt ID: (Human) Q01196
Entrez Gene ID: (Human) 861
regulatory region DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
ATP binding
DNA-binding transcription factor
immunoglobulin fold transcription factor
Runt transcription factor
gene-specific transcriptional regulator
ossification
chondrocyte differentiation
transcription from RNA polymerase II promoter
hemopoiesis
positive regulation of granulocyte differentiation
positive regulation of interleukin-2 production
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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