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CD15 Antibody (755-0158-94) in IHC (P)
Immunohistochemical analysis of CD15 was performed on formalin-fixed paraffin-embedded human tonsil (high expression) and human breast (low expression) tissues. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 10 min, followed by a 5 min cool down, a 1 min wash with ddH2O, and then transferred to 1X PBS. Tissues were permeabilize... View More
Product Details
755-0158-94
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MMA
Conjugate
Excitation/Emission Max
590/618 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The MMA monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
Applications Reported: This MMA antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of paraffin-embedded tissue.
Applications Tested: This MMA antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue with high pH antigen retrieval at 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1
Gene Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1
UniProt ID: (Human) P22083
Entrez Gene ID: (Human) 2526
carbohydrate metabolic process
protein glycosylation
protein N-linked glycosylation
protein O-linked glycosylation
sphingolipid metabolic process
glycosphingolipid biosynthetic process
inflammatory response
oligosaccharide metabolic process
oligosaccharide biosynthetic process
fucosylation
L-fucose catabolic process
lymphocyte migration into lymph node
Lewis x epitope biosynthetic process
positive regulation of neutrophil migration
regulation of leukocyte cell-cell adhesion
positive regulation of leukocyte tethering or rolling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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