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Collagen IV Antibody (757-9871-82) in IHC (P)
Immunohistochemical analysis of Collagen IV was performed on formalin-fixed paraffin-embedded human tonsil tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 10 minutes, followed by a 5-min cool down and a 5-min wash with ddH2O. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30 mins and blocked with 3% BSA/... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
757-9871-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
1042
Conjugate
Alexa Fluor™ Plus 750
Alexa Fluor™ Plus 750
Alexa Fluor™ Plus 750
Excitation/Emission Max
760/788 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture's syndrome.
Applications Reported: This 1042 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue sections.
Applications Tested: This 1042 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a matrix-derived inhibitor of angiogenesis and tumor growth; a3(IV)NC1; arresten; canstatin; COL4A1 NC1 domain; COL4A2 NC1 domain; COL4A3 NC1 domain; Collagen 4; collagen alpha-2 type IV; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen type IV alpha 5 chain; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; contains COL4A1 NC1 domain; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); matrix-derived inhibitor of angiogenesis and tumor growth; non-collagenous 1 domain from alpha 1 chain of type IV collagen; pro-alpha-1(IV); procollagen alpha-1 type IV; soluble Col4A1 protein; structural non-collagenous NC1 domain; a6[IV]NC1; tumstatin; Type IV Collagen; unnamed protein product
Gene Aliases: ASLN; ATS; ATS1; ATS2; ATS3; ATS3A; ATS3B; BFH; BFH1; BFH2; BSVD; BSVD1; BSVD2; BSVD2A; BSVD2B; CA44; CA54; COL4A1s; CXDELq22.3; DELXq22.3; DFNX6; ICH; PADMAL; POREN2; RATOR
UniProt ID: (Human) P02462, (Human) P08572, (Human) Q01955, (Human) P53420, (Human) P29400, (Human) Q14031
Entrez Gene ID: (Human) 1282, (Human) 1284, (Human) 1285, (Human) 1286, (Human) 1287, (Human) 1288
extracellular matrix organization
collagen fibril organization
collagen-activated tyrosine kinase receptor signaling pathway
patterning of blood vessels
brain development
epithelial cell differentiation
blood vessel morphogenesis
retinal blood vessel morphogenesis
renal tubule morphogenesis
basement membrane organization
glomerular basement membrane development
transcription, DNA-templated
response to activity
negative regulation of angiogenesis
endodermal cell differentiation
cellular response to transforming growth factor beta stimulus
cell surface receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
endothelial cell apoptotic process
negative regulation of vascular endothelial cell proliferation
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