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Invitrogen
A-Raf Polyclonal Antibody
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Product Details
PA5-21354
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Amount
85 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (85%), Xenopus laevis (86%), Pig (99%), Sheep (99%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; Oncogene ARAF1; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; raf-related oncogene; Ras-binding protein DA-Raf; RP1-230G1.1; Serine/threonine-protein kinase A-Raf; unnamed protein product; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein
Gene Aliases: 1200013E08Rik; A-RAF; ARAF; ARAF1; AW495444; PKS; PKS2; RAFA1
UniProt ID: (Human) P10398, (Mouse) P04627
Entrez Gene ID: (Human) 369, (Mouse) 11836
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
metal ion binding
protein serine kinase activity
receptor signaling protein activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
signal transduction
regulation of TOR signaling
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of peptidyl-serine phosphorylation
protein modification process
negative regulation of apoptotic process
activation of MAPKK activity
protein phosphorylation
phosphorylation
intracellular signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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