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GenScript
NWSHPQFEK Tag Monoclonal Antibody (5A9F9), Biotin, THE™
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NWSHPQFEK Tag Antibody (A01737-100) in WB
Western blot analysis of N-terminal and C-terminal Strep II tagged fusion protein using THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse (GenScript, A01737, 1 µg/mL). The signal was developed with Streptaidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensor THE™ HRP Substrate Kit (GenScript, L00221).
Product Details
A01737-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5A9F9
Immunogen
NWSHPQFEK peptide conjugated to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 10mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.GenScript THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
Target Information
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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