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Product Details
14304-1-AP
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ACK1 Fusion Protein Ag5419 (1-385 aa encoded by BC028164) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.13 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Product Specific Information
Immunogen sequence: MQPEEGTGW LLELLSEVQL QQYFLRLRDD LNVTRLSHFE YVKNEDLEKI GMGRPGQRRL WEAVKRRKAL CKRKSWMSKV FSGKRLEAEF PPHHSQSTFR KTSPAPGGPA GEGPLQSLTC LIGEKDLRLL EKLGDGSFGV VRRGEWDAPS GKTVSVAVKC LKPDVLSQPE AMDDFIREVN AMHSLDHRNL IRLYGVVLTP PMKMVTELAP LGSLLDRLRK HQGHFLLGTL SRYAVQVAEG MGYLESKRFI HRDLAARNLL LATRDLVKIG DFGLMRALPQ NDDHYVMQEH RKVPFAWCAP ESLKTRTFSH ASDTWMFGVT LWEMFTYGQE PWIGLNGSQI LHKIDKEGER LPRPEDCPQD IYNVMVQCWA HKPEDRPTFV ALRDFL (1-385 aa encoded by B C028164)
Target Information
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACK-1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; Cdc42 GTPase-inhibiting protein; Non-receptor protein tyrosine kinase Ack; P21cdc42Hs kinase; tnk2 {ECO:0000250|UniProtKB:Q07912}; Tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2
Gene Aliases: ACK; ACK-1; ACK1; Cdgip; p21cdc42Hs; Pyk1; TNK2
UniProt ID: (Human) Q07912, (Mouse) O54967, (Rat) Q5U2X5
Entrez Gene ID: (Human) 10188, (Mouse) 51789, (Rat) 303882
protein phosphorylation
endocytosis
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
cell differentiation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
innate immune response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of clathrin-mediated endocytosis
cell surface receptor signaling pathway
small GTPase mediated signal transduction
negative regulation of GTPase activity
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
GTPase inhibitor activity
epidermal growth factor receptor binding
protein binding
ATP binding
identical protein binding
metal ion binding
WW domain binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
hormone receptor binding
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