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Agrisera
ACT/Actin Polyclonal Antibody
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FIGURE: 1 / 4
ACT/Actin Antibody (AS13-2640) in ICC/IF
Type of material: isolated plant protoplasts from Arabidopsis thaliana wild type Fixation: formaldehyde Methanol, 37°C 3.2% paraformaldehyde in W 5 buffer (150 µm M Na Cl, 125 µm M Ca Cl 2,5 µm M KCl, 2 µm M MES p H 5.7), fixed overnight at +4ºC Hydrophilization: no Cell wall digestion: yes Membrane permeabilization: DMSO-IGEPA Antigen retrieval: no Blocking buffer: 2% BSA in 1 X Phosphate Buffered Sali... View More
Product Details
AS13-2640
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Algae,
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Agostis stolonifera cv. Penncross™,Arabidopsis thaliana, Brassica napus, Cucumis sativus, Cyanthobasis fruticulosa, Cynara cardunculus, Fragaria x ananassa,, Glycine max, Hordeum vulgare, Nicotiana tabacum, Odontarrhena lesbiaca, Petrosimonia nigdeensis, Phaseolus vulgaris, Phaeodactylum tricornutum, Phoenix dactylifera, Picrorhiza kurroa,Salsola grandis, Salsola tragus, Setaria italica, Solanum tuberosum, Triticum aestivum, Vigna unguiculata, Zea mays
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACT2; actin 2; actin 7; alpha-sma; AtACT7; Atcb; DEFORMED ROOT HAIRS 1; DER1; ENHANCER OF LRX1 2; ENL2; FIZ2; FRIZZY AND KINKED SHOOTS 2; LIGHT STRESS-REGULATED 2; LSR2; MYH9.2; MYH9_2; pan actin; RP5-1068B5.2
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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