Proteintech

ACTN4 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (22)

ACTN4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

ACTN4 Antibody (19096-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using ACTN4 antibody 19096-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ACTN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ACTN4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19096-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ACTN4 Fusion Protein Ag13546 (612-911 aa encoded by BC005033)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates.

Immunogen sequence: TTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL RRQFASQANV VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY KPNLDLLEQQ HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV ENQILTRDAK GISQEQMQEF RASFNHFDKD HGGALGPEEF KACLISLGYD VENDRQGEAE FNRIMSLVDP NHSGLVTFQA FIDFMSRETT DTDTADQVIA SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS FSTALYGESD L (612-911 aa encoded by BC005033)

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GIPC3 couples to MYO6 and PDZ domain proteins, and shapes the hair cell apical region.

Journal of cell science

Chatterjee P,Morgan CP,Krey JF,Benson C,Goldsmith J,Bateschell M,Ricci AJ,Barr-Gillespie PG

Mon May 15 00:00:00 EDT 2023

GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region.

bioRxiv : the preprint server for biology

Chatterjee P,Morgan CP,Krey JF,Benson C,Goldsmith J,Bateschell M,Ricci AJ,Barr-Gillespie PG

Wed Mar 01 00:00:00 EST 2023

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Cancer immunology, immunotherapy : CII

Chen X,Du Z,Huang M,Wang D,Fong WP,Liang J,Fan L,Wang Y,Yang H,Chen Z,Hu M,Xu R,Li Y

Tue Mar 01 00:00:00 EST 2022

The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.

Molecular biology of the cell

Kollimada S,Senger F,Vignaud T,Théry M,Blanchoin L,Kurzawa L

Thu Aug 19 00:00:00 EDT 2021

Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Reproductive biology and endocrinology : RB&E

Peng W,Liu Y,Qi H,Li Q

Tue Mar 23 00:00:00 EDT 2021

Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Reproductive biology and endocrinology : RB&E

Peng W,Liu Y,Qi H,Li Q

Tue Mar 23 00:00:00 EDT 2021

Protein tyrosine phosphatase 1B(PTP1B) promotes melanoma cells progression through Src activation.

Bioengineered

Wang Q,Pan Y,Zhao L,Qi F,Liu J

Wed Dec 01 00:00:00 EST 2021

NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.

Cell death & disease

Chen W,Wang W,Sun X,Xie S,Xu X,Liu M,Yang C,Li M,Zhang W,Liu W,Wang L,Zhou T,Yang Y

Tue Jul 14 00:00:00 EDT 2020

Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.

Journal of extracellular vesicles

Jiang W,Ma P,Deng L,Liu Z,Wang X,Liu X,Long G

Sat Oct 24 00:00:00 EDT 2020

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng W,Tong C,Li L,Huang C,Ran Y,Chen X,Bai Y,Liu Y,Zhao J,Tan B,Luo X,Wang H,Wen L,Zhang C,Zhang H,Ding Y,Qi H,Baker PN

Wed May 01 00:00:00 EDT 2019

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng W,Tong C,Li L,Huang C,Ran Y,Chen X,Bai Y,Liu Y,Zhao J,Tan B,Luo X,Wang H,Wen L,Zhang C,Zhang H,Ding Y,Qi H,Baker PN

Wed May 01 00:00:00 EDT 2019

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng W,Tong C,Li L,Huang C,Ran Y,Chen X,Bai Y,Liu Y,Zhao J,Tan B,Luo X,Wang H,Wen L,Zhang C,Zhang H,Ding Y,Qi H,Baker PN

Wed May 01 00:00:00 EDT 2019

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng W,Tong C,Li L,Huang C,Ran Y,Chen X,Bai Y,Liu Y,Zhao J,Tan B,Luo X,Wang H,Wen L,Zhang C,Zhang H,Ding Y,Qi H,Baker PN

Wed May 01 00:00:00 EDT 2019

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Journal of cellular biochemistry

Zhong J,Lan C,Zhang C,Yang Y,Chen WX,Zhang KY,Zhao HL,Fang XY,Li HH,Tan L,Wang P,Ge HF,Hu R,Feng H

Mon Jul 01 00:00:00 EDT 2019

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Journal of cellular biochemistry

Zhong J,Lan C,Zhang C,Yang Y,Chen WX,Zhang KY,Zhao HL,Fang XY,Li HH,Tan L,Wang P,Ge HF,Hu R,Feng H

Mon Jul 01 00:00:00 EDT 2019

Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.

Frontiers in cellular and infection microbiology

Fu J,Li L,Yang X,Yang R,Amjad N,Liu L,Tan C,Chen H,Wang X

Tue Sep 10 00:00:00 EDT 2019

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Scientific reports

Ge H,Yu A,Chen J,Yuan J,Yin Y,Duanmu W,Tan L,Yang Y,Lan C,Chen W,Feng H,Hu R

Tue Nov 22 00:00:00 EST 2016

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Scientific reports

Ge H,Yu A,Chen J,Yuan J,Yin Y,Duanmu W,Tan L,Yang Y,Lan C,Chen W,Feng H,Hu R

Tue Nov 22 00:00:00 EST 2016

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Scientific reports

Ge H,Yu A,Chen J,Yuan J,Yin Y,Duanmu W,Tan L,Yang Y,Lan C,Chen W,Feng H,Hu R

Tue Nov 22 00:00:00 EST 2016

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Scientific reports

Ge H,Yu A,Chen J,Yuan J,Yin Y,Duanmu W,Tan L,Yang Y,Lan C,Chen W,Feng H,Hu R

Tue Nov 22 00:00:00 EST 2016

Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Wang MC,Chang YH,Wu CC,Tyan YC,Chang HC,Goan YG,Lai WW,Cheng PN,Liao PC

Sun Feb 01 00:00:00 EST 2015

Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Wang MC,Chang YH,Wu CC,Tyan YC,Chang HC,Goan YG,Lai WW,Cheng PN,Liao PC

Sun Feb 01 00:00:00 EST 2015

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Amino acids

Bai C,Tang S,Bai C,Chen X

Tue Apr 01 00:00:00 EDT 2014

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Amino acids

Bai C,Tang S,Bai C,Chen X

Tue Apr 01 00:00:00 EDT 2014

α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.

PloS one

Hodges JL,Vilchez SM,Asmussen H,Whitmore LA,Horwitz AR

Mon Mar 30 00:00:00 EDT 2015

α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.

PloS one

Hodges JL,Vilchez SM,Asmussen H,Whitmore LA,Horwitz AR

Mon Mar 30 00:00:00 EDT 2015

MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

Oncogene

Agarwal N,Adhikari AS,Iyer SV,Hekmatdoost K,Welch DR,Iwakuma T

Thu Jan 24 00:00:00 EST 2013

MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

Oncogene

Agarwal N,Adhikari AS,Iyer SV,Hekmatdoost K,Welch DR,Iwakuma T

Thu Jan 24 00:00:00 EST 2013

Bioinformatics

Protein Aliases: actinin-Alpha-4; alpha actinin 4; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; Non-muscle alpha-actinin 4

View more View less

Gene Aliases: ACTININ-4; ACTN4; C77391; FSGS; FSGS1

View more View less

UniProt ID: (Human) O43707, (Mouse) P57780, (Rat) Q9QXQ0

View more View less

Entrez Gene ID: (Human) 81, (Mouse) 60595, (Rat) 63836

View more View less

Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding nucleoside binding actin binding integrin binding calcium ion binding protein binding ligand-dependent nuclear receptor transcription coactivator activity chromatin DNA binding nuclear hormone receptor binding protein homodimerization activity retinoic acid receptor binding ion channel binding poly(A) RNA binding protein N-terminus binding actin filament binding protein complex binding metal ion binding

Process(es)

transport protein transport peroxisome proliferator activated receptor signaling pathway regulation of apoptotic process retinoic acid receptor signaling pathway positive regulation of pinocytosis actin filament bundle assembly negative regulation of cellular component movement positive regulation of cellular component movement bicellular tight junction assembly protein localization to bicellular tight junction regulation of nucleic acid-templated transcription response to hypoxia platelet degranulation vesicle transport along actin filament positive regulation of cell migration positive regulation of sodium:proton antiporter activity negative regulation of substrate adhesion-dependent cell spreading positive regulation of NIK/NF-kappaB signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ACTN4 Polyclonal Antibody

ACTN4 Antibody (19096-1-AP) in ICC/IF

Product Details

19096-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!