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Invitrogen
ACTR1A Polyclonal Antibody
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FIGURE: 1 / 7
ACTR1A Antibody (PA5-114822) in ICC/IF
Immunocytochemistry analysis of ACTR1A in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114822) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114822
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACTR1A(Accession P61163), corresponding to amino acid residues I180-K230.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ACTR1A.
Target Information
This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin-related protein 1 alpha; actin-related protein 1 homolog A; Actin-RPV; actr1a {ECO:0000250|UniProtKB:P61163}; Alpha-centractin; ARP1; ARP1 actin-related protein 1 homolog A, centractin alpha; Arp1 centractin alpha; Arp1 contractin alpha; Centractin; Centrosome-associated actin homolog; FLJ52695; FLJ52800; FLJ55002; yeast homolog A
Gene Aliases: ACTR1A; alpha-Arp1; ARP1; Arp1A; CTRN1
UniProt ID: (Human) P61163, (Mouse) P61164, (Rat) P85515
Entrez Gene ID: (Human) 10121, (Mouse) 54130, (Rat) 294010
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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