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          • Primary Antibodies ›
          • ADO Antibodies

          Invitrogen

          ADO Polyclonal Antibody, DyLight™ 488

          View all (3) ADO antibodies

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          Cite ADO Polyclonal Antibody, DyLight™ 488

          Additional Information:
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          • Antibody Testing Data (1)
          ADO Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          ADO Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          ADO Antibody (PA5-78732) in Flow

          Flow Cytometry of ADO in A549 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum and then incubated with ADO Polyclonal Antibody, DyLight™ 488 (Product # PA5-78732) at a dilution of 1 μg (per 1x10^6 cells) for 30 min at 20°C. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          ADO Antibody in Flow Cytometry (Flow)
          ADO Polyclonal Antibody, DyLight™ 488

          Product Details

          PA5-78732

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived human ADO recombinant protein (Position: E49-E261).
          3D Epitope / Immunogen

          Conjugate

          DyLight™ 488 DyLight™ 488 DyLight™ 488

          Excitation/Emission Max

          492/519 nm View spectra spectra

          Form

          Liquid

          Concentration

          200 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2745848

          Product Specific Information

          Positive Control - Flow: A549 cell.

          Target Information

          Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-amino ethane thiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase; unnamed protein product

          View more View less

          Gene Aliases: ADO; C10orf22

          View more View less

          UniProt ID: (Human) Q96SZ5

          View more View less

          Entrez Gene ID: (Human) 84890

          View more View less

          Function(s)
          iron ion binding protein binding oxidoreductase activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen metal ion binding cysteamine dioxygenase activity dioxygenase activity
          Process(es)
          cellular response to hypoxia
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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