Proteintech

AFG3L2 Polyclonal Antibody

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AFG3L2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

AFG3L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14631-1-AP ( AFG3L2 Antibody) at dilution of 1:200 (under 40x lens).

AFG3L2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

AFG3L2 Antibody in Immunoprecipitation (IP) — IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

Product Details

14631-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AFG3L2 Fusion Protein Ag6209 (449-797 aa encoded by BC065016) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.36 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: ILAGTNRPD ILDPALLRPG RFDRQIFIGP PDIKGRASIF KVHLRPLKLD STLEKDKLAR KLASLTPGFS GADVANVCNE AALIAARHLS DSINQKHFEQ AIERVIGGLE KKTQVLQPEE KKTVAYHEAG HAVAGWYLEH ADPLLKVSII PRGKGLGYAQ YLPKEQYLYT KEQLLDRMCM TLGGRVSEEI FFGRITTGAQ DDLRKVTQSA YAQIVQFGMN EKVGQISFDL PRQGDMVLEK PYSEATARLI DDEVRILIND AYKRTVALLT EKKADVEKVA LLLLEKEVLD KNDMVELLGP RPFAEKSTYE EFVEGTGSLD EDTSLPEGLK DWNKEREKEK EEPPGEKVAN (449-797 aa encoded by BC065016)

Target Information

AFG3L2 is a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. AFG3L2 gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Cell reports

Jian F,Chen D,Chen L,Yan C,Lu B,Zhu Y,Chen S,Shi A,Chan DC,Song Z

Tue Jun 05 00:00:00 EDT 2018

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 EDT 2017

Proteolytic control of the mitochondrial calcium uniporter complex.

Proceedings of the National Academy of Sciences of the United States of America

Tsai CW,Wu Y,Pao PC,Phillips CB,Williams C,Miller C,Ranaghan M,Tsai MF

Tue Apr 25 00:00:00 EDT 2017

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

The Journal of cell biology

Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

Mon Oct 26 00:00:00 EDT 2015

Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.

Clinical science (London, England : 1979)

Gorman GS,Blakely EL,Hornig-Do HT,Tuppen HA,Greaves LC,He L,Baker A,Falkous G,Newman J,Trenell MI,Lecky B,Petty RK,Turnbull DM,McFarland R,Taylor RW

Mon Jun 01 00:00:00 EDT 2015

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.

Brain : a journal of neurology

Pfeffer G,Gorman GS,Griffin H,Kurzawa-Akanbi M,Blakely EL,Wilson I,Sitarz K,Moore D,Murphy JL,Alston CL,Pyle A,Coxhead J,Payne B,Gorrie GH,Longman C,Hadjivassiliou M,McConville J,Dick D,Imam I,Hilton D,Norwood F,Baker MR,Jaiser SR,Yu-Wai-Man P,Farrell M,McCarthy A,Lynch T,McFarland R,Schaefer AM,Turnbull DM,Horvath R,Taylor RW,Chinnery PF

Thu May 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: AFG3 ATPase family gene 3-like 2; AFG3 ATPase family member 3-like 2; AFG3 like AAA ATPase 2; AFG3(ATPase family gene 3)-like 2; AFG3-like AAA ATPase 2; AFG3-like protein 2; ATPase family gene 3, yeast; FLJ25993; Paraplegin-like protein

Gene Aliases: 2310036I02Rik; AFG3L2; AW260507; Emv66; par; SCA28; SPAX5

UniProt ID: (Human) Q9Y4W6, (Mouse) Q8JZQ2

Entrez Gene ID: (Human) 10939, (Mouse) 69597, (Rat) 307350

Molecular Function: metalloprotease