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AFG3L2 Polyclonal Antibody

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Antibody Testing Data (17)

FIGURE: 1 / 17

AFG3L2 Antibody (14631-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 14631-1-AP) AFG3L2 antibody at dilution of 1:8,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

AFG3L2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

AFG3L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14631-1-AP ( AFG3L2 Antibody) at dilution of 1:200 (under 40x lens).

AFG3L2 Antibody in Immunoprecipitation (IP) — IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

Product Details

14631-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AFG3L2 Fusion Protein Ag6209 (449-797 aa encoded by BC065016)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: ILAGTNRPD ILDPALLRPG RFDRQIFIGP PDIKGRASIF KVHLRPLKLD STLEKDKLAR KLASLTPGFS GADVANVCNE AALIAARHLS DSINQKHFEQ AIERVIGGLE KKTQVLQPEE KKTVAYHEAG HAVAGWYLEH ADPLLKVSII PRGKGLGYAQ YLPKEQYLYT KEQLLDRMCM TLGGRVSEEI FFGRITTGAQ DDLRKVTQSA YAQIVQFGMN EKVGQISFDL PRQGDMVLEK PYSEATARLI DDEVRILIND AYKRTVALLT EKKADVEKVA LLLLEKEVLD KNDMVELLGP RPFAEKSTYE EFVEGTGSLD EDTSLPEGLK DWNKEREKEK EEPPGEKVAN (449-797 aa encoded by BC065016)

Target Information

AFG3L2 is a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. AFG3L2 gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Li Y,Huang D,Jia L,Shangguan F,Gong S,Lan L,Song Z,Xu J,Yan C,Chen T,Tan Y,Liu Y,Huang X,Suzuki CK,Yang Z,Yang G,Lu B

Tue Jun 20 00:00:00 EDT 2023

The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response.

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Chamseddine D,Mahmud SA,Westfall AK,Castoe TA,Berg RE,Pellegrino MW

Tue Dec 13 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

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Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 EST 2022

Leber's hereditary optic neuropathy-associated ND6 14484T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.

Human molecular genetics

Liang M,Ji Y,Zhang L,Wang X,Hu C,Zhang J,Zhu Y,Mo JQ,Guan MX

Thu Sep 29 00:00:00 EDT 2022

Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.

Molecular cell

Tsai CW,Rodriguez MX,Van Keuren AM,Phillips CB,Shushunov HM,Lee JE,Garcia AM,Ambardekar AV,Cleveland JC,Reisz JA,Proenza C,Chatfield KC,Tsai MF

Thu Oct 06 00:00:00 EDT 2022

Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.

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Tesfay L,Paul BT,Hegde P,Brewer M,Habbani S,Jellison E,Moore T,Wu H,Torti SV,Torti FM

Sat Nov 19 00:00:00 EST 2022

Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis.

Human molecular genetics

Ng KY,Richter U,Jackson CB,Seneca S,Battersby BJ

Fri Apr 22 00:00:00 EDT 2022

A novel mutation located in the intermembrane space domain of AFG3L2 causes dominant optic atrophy through decreasing the stability of the encoded protein.

Cell death discovery

Yang L,Jin X,Li Y,Guo Q,Yang M,You Y,Yao S,Zhang X,Wang Z,Lei B

Mon Aug 15 00:00:00 EDT 2022

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

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Sun D,Niu Z,Zheng HX,Wu F,Jiang L,Han TQ,Wei Y,Wang J,Jin L

Mon Mar 07 00:00:00 EST 2022

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNA^His mutation.

The Journal of biological chemistry

Gong S,Wang X,Meng F,Cui L,Yi Q,Zhao Q,Cang X,Cai Z,Mo JQ,Liang Y,Guan MX

Fri Jan 24 00:00:00 EST 2020

Expanding the clinical and genetic heterogeneity of SPAX5.

Annals of clinical and translational neurology

Dosi C,Galatolo D,Rubegni A,Doccini S,Pasquariello R,Nesti C,Sicca F,Barghigiani M,Battini R,Tessa A,Santorelli FM

Wed Apr 01 00:00:00 EDT 2020

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Nucleic acids research

Chen D,Zhang Z,Chen C,Yao S,Yang Q,Li F,He X,Ai C,Wang M,Guan MX

Tue Jun 04 00:00:00 EDT 2019

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Life science alliance

Richter U,Ng KY,Suomi F,Marttinen P,Turunen T,Jackson C,Suomalainen A,Vihinen H,Jokitalo E,Nyman TA,Isokallio MA,Stewart JB,Mancini C,Brusco A,Seneca S,Lombès A,Taylor RW,Battersby BJ

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Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca²⁺ handling in HeLa cells.

Biochimica et biophysica acta. Molecular cell research

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Proteolytic control of the mitochondrial calcium uniporter complex.

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Tsai CW,Wu Y,Pao PC,Phillips CB,Williams C,Miller C,Ranaghan M,Tsai MF

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Proteolytic control of the mitochondrial calcium uniporter complex.

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Tsai CW,Wu Y,Pao PC,Phillips CB,Williams C,Miller C,Ranaghan M,Tsai MF

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Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 EDT 2017

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 EDT 2017

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

The Journal of cell biology

Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

Mon Oct 26 00:00:00 EDT 2015

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

The Journal of cell biology

Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

Mon Oct 26 00:00:00 EDT 2015

Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.

Clinical science (London, England : 1979)

Gorman GS,Blakely EL,Hornig-Do HT,Tuppen HA,Greaves LC,He L,Baker A,Falkous G,Newman J,Trenell MI,Lecky B,Petty RK,Turnbull DM,McFarland R,Taylor RW

Mon Jun 01 00:00:00 EDT 2015

Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.

Clinical science (London, England : 1979)

Gorman GS,Blakely EL,Hornig-Do HT,Tuppen HA,Greaves LC,He L,Baker A,Falkous G,Newman J,Trenell MI,Lecky B,Petty RK,Turnbull DM,McFarland R,Taylor RW

Mon Jun 01 00:00:00 EDT 2015

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.

Brain : a journal of neurology

Pfeffer G,Gorman GS,Griffin H,Kurzawa-Akanbi M,Blakely EL,Wilson I,Sitarz K,Moore D,Murphy JL,Alston CL,Pyle A,Coxhead J,Payne B,Gorrie GH,Longman C,Hadjivassiliou M,McConville J,Dick D,Imam I,Hilton D,Norwood F,Baker MR,Jaiser SR,Yu-Wai-Man P,Farrell M,McCarthy A,Lynch T,McFarland R,Schaefer AM,Turnbull DM,Horvath R,Taylor RW,Chinnery PF

Thu May 01 00:00:00 EDT 2014

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.

Brain : a journal of neurology

Thu May 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: AFG3 ATPase family gene 3-like 2; AFG3 ATPase family member 3-like 2; AFG3 like AAA ATPase 2; AFG3(ATPase family gene 3)-like 2; AFG3-like AAA ATPase 2; AFG3-like protein 2; ATPase family gene 3, yeast; FLJ25993; Paraplegin-like protein

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Gene Aliases: 2310036I02Rik; AFG3L2; AW260507; Emv66; par; SCA28; SPAX5

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UniProt ID: (Human) Q9Y4W6, (Mouse) Q8JZQ2

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Entrez Gene ID: (Human) 10939, (Mouse) 69597, (Rat) 307350

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Molecular Function: metalloprotease

Function(s)

protein complex assembly axonogenesis neuromuscular junction development mitochondrial fusion nerve development mitochondrial protein processing regulation of multicellular organism growth cristae formation myelination protein import into mitochondrial intermembrane space muscle fiber development righting reflex proteolysis mitochondrion organization obsolete death

Process(es)

ATP-dependent peptidase activity metalloendopeptidase activity protein binding ATP binding metallopeptidase activity zinc ion binding unfolded protein binding nucleotide binding peptidase activity hydrolase activity metal ion binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AFG3L2 Polyclonal Antibody

AFG3L2 Antibody (14631-1-AP) in WB

Product Details

14631-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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