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Invitrogen
AGO1/AGO2 Polyclonal Antibody
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FIGURE: 1 / 2
AGO1/AGO2 Antibody (PA5-40075) in WB
Whole cell extracts of U2OS cells (40 µg) were analyzed by Western Blot using a AGO1 + AGO2 polyclonal antibody (Product # PA5-40075) at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (kDa) is shown on the left.
Product Details
PA5-40075
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of both EIF2C1 and EIF2C2 corresponding to human IEF2C1/2 (eukaryotic translation initiation factor 2C1 and 2).
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
IEF2C1 and 2 are members of the Argonaute family. These proteins are involved in short-interfering-RNA-mediated gene silencing. This process is mediated by the RISC (RNA induced silencing complex), a multicomponent nuclease that is able to degrade double stranded RNAs. The mRNA degradation is triggered by the association of specific siRNAs with their homologous mRNAs. IEF2C2 has been shown to be essential for embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: argonaute 1; argonaute 2; Argonaute RISC catalytic component 1; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; CTA-204B4.6; eIF-2C 1; eIF-2C 2; eIF2C 1; eIF2C 2; Eukaryotic translation initiation factor 2C 1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 1; eukaryotic translation initiation factor 2C, 2; Golgi Endoplasmic Reticulum protein 95 kDa; hAgo2; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer; Putative RNA-binding protein Q99
Gene Aliases: AGO1; AGO2; EIF2C; EIF2C1; EIF2C2; GERP95; hAgo1; Q10; Q99
UniProt ID: (Human) Q9UL18, (Human) Q9UKV8
Entrez Gene ID: (Human) 26523, (Human) 27161
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
core promoter binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
endoribonuclease activity
protein binding
miRNA binding
poly(A) RNA binding
RNA 7-methylguanosine cap binding
mRNA binding
translation initiation factor activity
protein C-terminus binding
siRNA binding
metal ion binding
endoribonuclease activity, cleaving siRNA-paired mRNA
endoribonuclease activity, cleaving miRNA-paired mRNA
translation initiation factor
nuclear-transcribed mRNA catabolic process
transcription, DNA-templated
Wnt signaling pathway, calcium modulating pathway
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol-mediated signaling
mRNA cleavage involved in gene silencing by siRNA
translation
translational initiation
post-embryonic development
siRNA loading onto RISC involved in RNA interference
negative regulation of translational initiation
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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