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AGO2 Antibody (PA5-72107) in IHC (P)
Immunohistochemistry analysis of AGO2 in formalin fixed and paraffin embedded human stomach tissue. Samples were incubated with AGO2 polyclonal antibody (Product # PA5-72107) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Product Details
PA5-72107
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 27-59 amino acids from the N-terminal region of human EIF2C2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2717961
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or “Slicer” activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute2; CTA-204B4.6; eIF-2C 2; eIF2C; eIF2C 1; eIF2C 2; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 2; hAgo1; hAgo2; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer
Gene Aliases: AGO2; EIF2C2; Q10
UniProt ID: (Human) Q9UKV8
Entrez Gene ID: (Human) 27161
RNA 7-methylguanosine cap binding
RNA polymerase II core binding
core promoter binding
double-stranded RNA binding
single-stranded RNA binding
mRNA binding
translation initiation factor activity
endoribonuclease activity
protein binding
protein C-terminus binding
siRNA binding
miRNA binding
poly(A) RNA binding
metal ion binding
endoribonuclease activity, cleaving siRNA-paired mRNA
endoribonuclease activity, cleaving miRNA-paired mRNA
translation initiation factor
transcription, DNA-templated
translation
translational initiation
Wnt signaling pathway, calcium modulating pathway
post-embryonic development
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
siRNA loading onto RISC involved in RNA interference
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
negative regulation of translational initiation
phosphatidylinositol-mediated signaling
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
RNA phosphodiester bond hydrolysis, endonucleolytic
mRNA cleavage involved in gene silencing by siRNA
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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