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Invitrogen
AGO2 Recombinant Rabbit Monoclonal Antibody (036)
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Product Details
MA5-29649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
036
Immunogen
Recombinant Mouse AGO2 protein (Met1-Ala860)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse AGO2.
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; eIF-2C 1; eIF-2C 2; eIF2C; eIF2C 1; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 2; hAgo1; mAgo2; Piwi/Argonaute family protein meIF2C2; Protein argonaute-1; Protein argonaute-2; Protein slicer
Gene Aliases: 1110029L17Rik; 2310051F07Rik; Ago2; AI225898; AL022874; AW546247; Eif2c2; ENSMUSG00000072493; Gerp95; Gm10365; Kiaa4215; mKIAA4215
UniProt ID: (Mouse) Q8CJG0
Entrez Gene ID: (Mouse) 239528
RNA 7-methylguanosine cap binding
RNA polymerase II core binding
core promoter binding
nucleic acid binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
mRNA binding
nuclease activity
endonuclease activity
endoribonuclease activity
protein binding
hydrolase activity
siRNA binding
miRNA binding
poly(A) RNA binding
metal ion binding
endoribonuclease activity, cleaving siRNA-paired mRNA
pre-miRNA binding
endoribonuclease activity, cleaving miRNA-paired mRNA
translation initiation factor
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA cleavage
regulation of translation
multicellular organism development
post-embryonic development
RNA secondary structure unwinding
miRNA metabolic process
positive regulation of gene expression
cell differentiation
gene silencing by RNA
pre-miRNA processing
siRNA loading onto RISC involved in RNA interference
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
negative regulation of translational initiation
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
RNA phosphodiester bond hydrolysis, endonucleolytic
mRNA cleavage involved in gene silencing by siRNA
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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