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Invitrogen
AGO2 Monoclonal Antibody (R.386.2)
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FIGURE: 1 / 1
AGO2 Antibody (MA5-14861) in WB
Western blot was performed using anti-AGO2 Monoclonal Antibody (R.386.2) (Product # MA5-14861) and a band at 97 kDa corresponding to AGO2 was observed across the cell lines tested and was increased upon LPS treatment in THP-1. An uncharacterized band (*) was also observed at ~20 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), Hep G2 (Lane 4), THP-1 (Lane 5), THP-1 treated with LPS (1 µg/mL for 24hrs) (Lane 6) and RAW 264.7 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-... View More
Product Details
MA5-14861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
R.386.2
Immunogen
Synthetic peptide corresponding to mouse Argonaute 2
Conjugate
Form
Liquid
Concentration
260 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AGO1; argonaute 2; argonaute 2, RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031}; cancer susceptibility candidate 7 (non-protein coding); eIF-2C 1; eIF-2C 2; eIF-2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eIF2C; eIF2C 1; eIF2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eukaryotic translation initiation factor 2C, 2; GERp95; golgi ER protein 95 kDa; hAgo1; long intergenic non-protein coding RNA 980; mAgo2; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer
Gene Aliases: 1110029L17Rik; 2310051F07Rik; AGO2; AI225898; AL022874; AW546247; CASC7; EIF2C2; ENSMUSG00000072493; Gerp95; Gm10365; Kiaa4215; LESKRES; LINC00980; mKIAA4215; PPD; Q10
UniProt ID: (Human) Q9UKV8, (Mouse) Q8CJG0
Entrez Gene ID: (Human) 27161, (Rat) 59117, (Mouse) 239528
RNA 7-methylguanosine cap binding
RNA polymerase II core binding
core promoter sequence-specific DNA binding
nucleic acid binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
mRNA binding
translation initiation factor activity
nuclease activity
endonuclease activity
endoribonuclease activity
protein binding
hydrolase activity
endoribonuclease activity, producing 5'-phosphomonoesters
siRNA binding
miRNA binding
mRNA 3'-UTR AU-rich region binding
metal ion binding
endoribonuclease activity, cleaving siRNA-paired mRNA
endoribonuclease activity, cleaving miRNA-paired mRNA
mRNA cap binding
molecular_function
poly(A) RNA binding
pre-miRNA binding
core promoter binding
translation initiation factor
regulation of transcription, DNA-templated
translation
translational initiation
regulation of translation
post-embryonic development
gene expression
miRNA metabolic process
positive regulation of gene expression
production of siRNA involved in RNA interference
gene silencing by RNA
pre-miRNA processing
cytoplasmic mRNA processing body assembly
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
negative regulation of amyloid precursor protein biosynthetic process
RNA stabilization
positive regulation of translation
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
negative regulation of translational initiation
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
small RNA loading onto RISC
regulation of synapse maturation
mRNA cleavage involved in gene silencing by siRNA
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
positive regulation of trophoblast cell migration
transcription, DNA-templated
mRNA cleavage
biological_process
cell differentiation
miRNA loading onto RISC involved in gene silencing by miRNA
RNA phosphodiester bond hydrolysis, endonucleolytic
multicellular organism development
RNA secondary structure unwinding
siRNA loading onto RISC involved in RNA interference
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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