|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide from near the center of human AGTR1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2.5 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control is mouse kidney tissue lysate.
PA5-20812 can be used with blocking peptide PEP-0926.
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion that acts through at least two types of receptors, AGTR1 and AGTR2. It is an important effector controlling blood pressure and volume in the cardiovascular system and plays a major role in the development of the mammalian kidney and urinary tract. AGTR1, the type 1 receptor, is thought to mediate the major cardiovascular effects of angiotensin II and may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. AGTR1 has recently been found to regulate the differentiation of bone marrow-derived monocyte lineage progenitors from hematopoietic stem cells, indicating the diversity of the roles of AGTR1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential expression and DNA methylation of angiotensin type 1A receptors in vascular tissues during genetic hypertension development.
PA5-20812 was used in western blot to study AT1aR expression and methylation during the development of hypertension
|Pei F,Wang X,Yue R,Chen C,Huang J,Huang J,Li X,Zeng C||Molecular and cellular biochemistry (402:1)||2015|