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Invitrogen
EIF2S1 Monoclonal Antibody (EIF2-alpha)
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FIGURE: 1 / 32
EIF2S1 Antibody (AHO0802) in ICC/IF
Immunofluorescence analysis of eIF2a was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with eIF2a Mouse Monoclonal Antibody at 1:250 dilution in1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for... View More
Product Details
AHO0802
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse,
Non-human primate,
Rat,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
EIF2-alpha
Immunogen
Recombinant human eIF-2-alpha.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes the alpha subunit of eukaryotic translation initiation factor 2 (EIF2-alpha). Recommended positive control are human Jurkat, CEM, and HeLa cells; mouse 3T3L1 cells; and rat PC-12 cells.
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; eIF2-alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A; translational initiation factor eIF-2, alpha subunit; unnamed protein product
Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1
UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6
Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665
mitophagy
translation
translational initiation
regulation of translation
negative regulation of translational initiation in response to stress
stress granule assembly
cellular response to amino acid starvation
cellular response to oxidative stress
cellular response to heat
cellular response to UV
response to endoplasmic reticulum stress
regulation of translation in response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
regulation of translational initiation in response to stress
mitochondrial respiratory chain supercomplex assembly
HRI-mediated signaling
response to kainic acid
response to manganese-induced endoplasmic reticulum stress
positive regulation of type B pancreatic cell apoptotic process
protein autophosphorylation
positive regulation of defense response to virus by host
mitophagy in response to mitochondrial depolarization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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