This Antibody was verified by KnockOut to ensure that the antibody binds to the antigen stated. View Details
In Western blot applications, this antibody detects a band at ~67kDa. A suggested positive control is K562 cell lysate.
PA5-19953 can be used with blocking peptide PEP-0078.
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
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Protein Aliases: anterior gradient homolog 1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; endoplasmic reticulum protein ERp19; endoplasmic reticulum resident protein 18; endoplasmic reticulum resident protein 19; endoplasmic reticulum thioredoxin superfamily member, 18 kDa; harlequin; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; protein disulfide isomerase family A, member 16; striatal apoptosis-inducing factor; testicular secretory protein Li 4; thioredoxin domain-containing protein 12; thioredoxin-like protein p19; UNQ713/PRO1376, AG1, AGR1, ERP18, ERP19, PDIA16, TLP19, hAG-1
Gene Aliases: AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8