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AKR1C4 Antibody (PA5-21501) in ICC/IF
AKR1C4 Polyclonal Antibody detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD3a protein stained by AKR1C4 Polyclonal Antibody (Product # PA5-21501) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-21501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 217 of HSD3a (Uniprot ID#P17516)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.83 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11153895
Product Specific Information
Recommended positive controls: HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; 3alpha-hydroxysteroid 3-dehydrogenase; Aldo-keto reductase family 1 member C4; CDR; Chlordecone reductase; chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4; DD-4; Dihydrodiol dehydrogenase 4; dihydrodiol dehydrogenase isozyme DD4; HAKRA; HGNC:387; MGC22581; type I 3-alpha-hydroxysteroid dehydrogenase
Gene Aliases: 3-alpha-HSD; AKR1C4; C11; CDR; CHDR; DD-4; DD4; HAKRA
UniProt ID: (Human) P17516
Entrez Gene ID: (Human) 1109
retinal dehydrogenase activity
aldo-keto reductase (NADP) activity
electron carrier activity
bile acid transmembrane transporter activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
androsterone dehydrogenase activity
chlordecone reductase activity
oxidoreductase
reductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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