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ALAS2 Antibody (PA5-121007) in WB
Western blot analysis of ALAS2 in extracts of various cell lines. Samples were incubated with ALAS2 Polyclonal antibody (Product # PA5-121007) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-121007
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human ALAS2 (NP_001033057.1).
Conjugate
Form
Liquid
Concentration
1.28 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914579
Product Specific Information
Positive test controls include: 293T, Mouse liver, Mouse spleen, Mouse lung. The target is usually found in the following locations: Mitochondrion matrix.
Immunogen sequence: VKTHQFLFGI GRCPILATQG PNCSQIHLKA TKAGGDSPSW AKGHCPFMLS ELQDGKSKIV QKAAPEVQED VKAFKTGNYV FSYDQFFRDK IMEKKQDHTY RVFKTVNRWA DAYPFAQHFS EASVASKDVS VWCSNDYLGM SRHPQVLQAT QETLQRHGAG AGGTRNISGT SKFHVELEQE LAELHQKDSA LLFSSCFVAN DSTLFTLAKI LPGCEIYSDA GNHASMIQGI RNSGAAKFVF RHNDPDHLKK LLEKSNPKIP KIVAFETVHS M
Target Information
ALAS2 specifies an erythroid-specific mitochondrially located enzyme. It catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia.The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-aminolevulinate synthase, erythroid-specific, mitochondrial; 5-aminolevulinic acid synthase 2; ALAS-E; Aminolevulinate synthase 2, delta; aminolevulinate, delta-, synthase 2; aminolevulinic acid synthase 2, erythroid; Delta-ALA synthase 2; delta-ALA synthetase; Delta-aminolevulinate synthase 2; erythroid-specific ALAS; erythroid-specific delta-aminolevulinate synthase ALAS-E; PRO2399; RP5-884M20.1
Gene Aliases: ALAS; Alas-2; ALAS-E; ALAS2; ALASE; ANH1; ASB; SIDBA1; XLDPP; XLEPP; XLSA
UniProt ID: (Human) P22557, (Rat) Q63147, (Mouse) P08680
Entrez Gene ID: (Human) 212, (Rat) 25748, (Mouse) 11656
response to hypoxia
protoporphyrinogen IX biosynthetic process
heme biosynthetic process
cellular iron ion homeostasis
erythrocyte differentiation
oxygen homeostasis
hemoglobin biosynthetic process
female pregnancy
lactation
response to lead ion
response to drug
porphyrin-containing compound metabolic process
metabolic process
biosynthetic process
tetrapyrrole biosynthetic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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