Invitrogen

ALDH1A1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ALDH1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ALDH1A1 in paraffin-embedded human colon carcinoma using ALDH1A1 Polyclonal Antibody (Product # PA5-95937) at a dilution of 1:100.

ALDH1A1 Antibody in Western Blot (WB) — Western Blot analysis of ALDH1A1 in extracts of various cell lines using ALDH1A1 Polyclonal Antibody (Product # PA5-95937) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-95937

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rodent
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to amino acids 250-350 of human ALDH1A1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.04 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.05% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2807739

Product Specific Information

Immunogen sequence: ELCQVEEGDK EDVDKAVKAA RQAFQIGSPW RTMDASERGR LLYKLADLIE RDRLLLATME SMNGGKLYSN AYLNDLAGCI KTLRYCAGWA DKIQGRTIPI; Positive Samples: A-549, HT-29, K-562, Mouse liver, Mouse lung, Rat lung; Cellular Location: Cytoplasm

Target Information

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation.

Cell reports

Zhang Q,Tombline G,Ablaeva J,Zhang L,Zhou X,Smith Z,Zhao Y,Xiaoli AM,Wang Z,Lin JR,Jabalameli MR,Mitra J,Nguyen N,Vijg J,Seluanov A,Gladyshev VN,Gorbunova V,Zhang ZD

PA5-95937 was used in Western Blot to report the evolutionary changes in the North American beaver gene coding sequences, copy numbers, and expression towards explaining its cancer-resistance and longevity.

Tue Nov 09 00:00:00 EST 2021

Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation.

Cell reports

Zhang Q,Tombline G,Ablaeva J,Zhang L,Zhou X,Smith Z,Zhao Y,Xiaoli AM,Wang Z,Lin JR,Jabalameli MR,Mitra J,Nguyen N,Vijg J,Seluanov A,Gladyshev VN,Gorbunova V,Zhang ZD

Tue Nov 09 00:00:00 EST 2021

Bioinformatics

Protein Aliases: 3-deoxyglucosone dehydrogenase; acetaldehyde dehydrogenase 1; alcohol dehydrogenase family 1, subfamily A1; alcohol dehydrogenase family 1, subfamily A2; aldehyde dehydrogenase 1 family, member A1; Aldehyde dehydrogenase 1 soluble; aldehyde dehydrogenase 1, liver cytosolic (class 1); aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase 1, subfamily A1; Aldehyde dehydrogenase 1A1; aldehyde dehydrogenase 2; Aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase family 1, member A1; aldehyde dehydrogenase family 1, subfamily A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH-E1; ALHDII; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; epididymis secretory sperm binding protein Li53e; MGC2318; RALDH 1; ralDH1; Retinal dehydrogenase 1; retinaldehyde dehydrogenase 1

Gene Aliases: Ahd-2; Ahd2; ALDC; Aldh; ALDH-E1; ALDH1; ALDH11; ALDH1A1; Aldh1a2; Aldh2; E1; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1

UniProt ID: (Human) P00352, (Mouse) P24549, (Rat) P51647

Entrez Gene ID: (Human) 216, (Mouse) 11668, (Rat) 24188

Molecular Function: dehydrogenase oxidoreductase metabolite interconversion enzyme