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Immunofluorescent analysis of ALDH7A1 showing staining in the mitochondria and nucleus of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using an ALDH7A1 polyclonal antibody (Product # PA5-29974) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 261 and 479 of Human ALDH7A1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29974 targets ALDH7A1 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-29974 immunogen is recombinant protein fragment corresponding to a region within amino acids 261 and 479 of Human ALDH7A1.
Antiquitin is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. Mutations in this gene cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride. Four additional human antiquitin-like sequences, all of which are pseudogenes, have also been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
D18Wsu181e, Atq1, PDE, ATQ1, EPD
P6c dehydrogenase, aldehyde dehydrogenase 7 family, member A1, alpha-AASA dehydrogenase, alpha-aminoadipic semialdehyde dehydrogenase, antiquitin-1, betaine aldehyde dehydrogenase, delta1-piperideine-6-carboxylate dehydrogenase, delta1-piperideine-6-carboxylate dehydrogenease, 26g turgor protein homolog, ATQ1, EPD, PDE