Invitrogen

ALG12 Polyclonal Antibody

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ALG12 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ALG12 in paraffin-embedded Human thyroid cancer tissue. Samples were probed using a ALG12 polyclonal antibody (Product # PA5-50316) at a dilution of 1/20.

ALG12 Antibody in Western Blot (WB) — Western blot analysis of ALG12 was performed by loading PC3 cell lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a ALG12 antibody (Product # PA5-50316) at a 1/200 dilution for 10 seconds, followed by a secondary antibody at 1/8000.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:100

Western Blot (WB)

1:200-1:1000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein corresponding to a region derived from internal residues of human ALG12, alpha-1,6-mannosyltransferase

Conjugate

Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2635769

Product Specific Information

The antibody detects endogenous levels of total ALG12 protein.

Target Information

This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: asparagine-linked glycosylation 12 homolog (S. cerevisiae, alpha-1,6-mannosyltransferase); asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase); asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog; Asparagine-linked glycosylation protein 12 homolog; dol-P-Man dependent alpha-1,6-mannosyltransferase; Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase; Dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase; dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-alpha-6-mannosyltransferase; hALG12; Mannosyltransferase ALG12 homolog; Membrane protein SB87

Gene Aliases: ALG12; CDG1G; ECM39; hALG12; PP14673

UniProt ID: (Human) Q9BV10

Entrez Gene ID: (Human) 79087

Molecular Function: glycosyltransferase transferase